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SPAG5通过调节细胞增殖和凋亡参与人类胶质瘤的发生。

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis.

作者信息

Wang Chunhong, Su Haiyang, Cheng Rui, Ji Hongming

机构信息

Department of Neurosurgery, Shanxi Medical University Shanxi Provincial People's Hospital, Taiyuan, China.

出版信息

Front Oncol. 2021 Nov 2;11:673780. doi: 10.3389/fonc.2021.673780. eCollection 2021.

DOI:10.3389/fonc.2021.673780
PMID:34796102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8592975/
Abstract

BACKGROUND

Glioma is the most frequent malignant primary brain tumor in adults.

OBJECTIVE

To explore the role of sperm-associated antigen 5 (SPAG5) in glioma.

METHODS

The association between SPAG5 expression and clinical features was investigated based on The Cancer Genome Atlas (TCGA) datasets. The function of SPAG5 in glioma was analyzed using U87 and U251 cells. Knockdown glioma cells were constructed by shRNA interference. qRT-PCR and Western blotting were used to measure the expression of SPAG5 and Cadherin 2 (CDH2). Cell proliferation and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, caspase 3/7 assay, and high-content screening (HCS) proliferation analysis and colony formation assay. Transwell assays and wound-healing assays were used to investigate cell migration and invasion.

RESULTS

The increased expression of SPAG5 was correlated with poor outcomes in glioma patients. Knocking down SPAG5 could inhibit the proliferation and colony formation and promoted the apoptosis of glioma cells. Knocking down SPAG5 could also inhibit cell migration and invasion and the expression of CDH2. Overexpression of CDH2 with SPAG5 depletion could restore the proliferation and inhibit the apoptosis of glioma cells, which also promoted cell migration and invasion.

CONCLUSIONS

SPAG5 is a promising prognostic factor and potential therapeutic target for clinical intervention in glioma.

摘要

背景

胶质瘤是成人中最常见的原发性恶性脑肿瘤。

目的

探讨精子相关抗原5（SPAG5）在胶质瘤中的作用。

方法

基于癌症基因组图谱（TCGA）数据集研究SPAG5表达与临床特征之间的关联。使用U87和U251细胞分析SPAG5在胶质瘤中的功能。通过shRNA干扰构建敲低胶质瘤细胞。采用qRT-PCR和蛋白质免疫印迹法检测SPAG5和钙黏蛋白2（CDH2）的表达。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、半胱天冬酶3/7法、高内涵筛选（HCS）增殖分析和集落形成试验检测细胞增殖和凋亡。采用Transwell试验和伤口愈合试验研究细胞迁移和侵袭。

结果

SPAG5表达增加与胶质瘤患者的不良预后相关。敲低SPAG5可抑制胶质瘤细胞的增殖和集落形成并促进其凋亡。敲低SPAG5还可抑制细胞迁移和侵袭以及CDH2的表达。在SPAG5缺失的情况下过表达CDH2可恢复胶质瘤细胞的增殖并抑制其凋亡，这也促进了细胞迁移和侵袭。

结论

SPAG5是一种有前景的预后因素，也是胶质瘤临床干预的潜在治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/0e5be03d4eb0/fonc-11-673780-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/8663d8d0a4e7/fonc-11-673780-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/0e5be03d4eb0/fonc-11-673780-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/9e80c4398b8f/fonc-11-673780-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/c1736ba0500d/fonc-11-673780-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/8663d8d0a4e7/fonc-11-673780-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e653/8592975/0e5be03d4eb0/fonc-11-673780-g008.jpg

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