[精子相关抗原5（SPAG5）在胃癌细胞恶性增殖中的生物学作用]

[Biological role of SPAG5 in the malignant proliferation of gastric cancer cells].

作者信息

Pang Y, Liu Y, Chen S, Zhang J, Zeng J, Pan Y, An J

机构信息

Medical College, Qinghai University, Xining, 810001, China.

Cancer Research Center, Beijing Chest Hospital, Capital Medical University, Beijing, 101149, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2024 Aug 20;44(8):1497-1507. doi: 10.12122/j.issn.1673-4254.2024.08.08.

DOI:10.12122/j.issn.1673-4254.2024.08.08

PMID:39276045

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11378056/

Abstract

OBJECTIVE

To analyze the expression of SPAG5 in gastric cancer tissues and its regulatory roles in gastric cancer cell growth.

METHODS

TCGA analysis, immunohistochemistry, and immunofluorescence staining were used to analyze the expression patterns of SPAG5 and MKi67 in gastric cancer and adjacent tissues. In gastric cancer AGS and MGC803 cells, the effects of lentivirus-mediated SPAG5 knockdown on cell growth and apoptosis were evaluated using Celigo, MTT, clone formation assays and flow cytometry.

RESULTS

Proteinatlas and TCGA database analysis suggested that SPAG5 was highly expressed in gastric cancer, and Kaplan-Meier analysis and GEPIA analysis showed high expressions of SPAG 5 in lung adenocarcinoma, breast cancer, hepatocellular carcinoma, pancreatic carcinoma, cervical cancer and bladder carcinoma. Immunohistochemistry revealed that SPAG5 was highly expressed in gastric cancer tissues ( < 0.001), and immunofluorescence colocalization analysis demonstrated a significant correlation between SPAG5 and MKI67 (=0.393, < 0.001). RT-qPCR and Western blotting showed that SPAG5 was highly expressed in MKN74, BGC823, MGC803, SGC7901 and AGS cells. In AGS and MGC803 cells, SPAG5 knockdown significantly inhibited proliferation and promoted apoptosis.

CONCLUSIONS

The expressions of SPAG5 and MKi67 are correlated in gastric cancer tissues, and SPAG5 knockdown inhibits the proliferation of gastric cancer cells. SPAG5 is associated with the prognosis of gastric cancer patients and may serve as a promising biomarker for gastric cancer.

摘要

目的

分析精子相关抗原5（SPAG5）在胃癌组织中的表达及其对胃癌细胞生长的调控作用。

方法

采用TCGA分析、免疫组织化学和免疫荧光染色法分析SPAG5和MKi67在胃癌及癌旁组织中的表达模式。在胃癌AGS和MGC803细胞中，使用Celigo、MTT、克隆形成实验和流式细胞术评估慢病毒介导的SPAG5敲低对细胞生长和凋亡的影响。

结果

蛋白质图谱和TCGA数据库分析表明，SPAG5在胃癌中高表达，Kaplan-Meier分析和GEPIA分析显示SPAG5在肺腺癌、乳腺癌、肝细胞癌、胰腺癌、宫颈癌和膀胱癌中高表达。免疫组织化学显示SPAG5在胃癌组织中高表达（<0.001），免疫荧光共定位分析表明SPAG5与MKI67之间存在显著相关性（=0.393，<0.001）。RT-qPCR和蛋白质印迹显示SPAG5在MKN74、BGC823、MGC803、SGC7901和AGS细胞中高表达。在AGS和MGC803细胞中，SPAG5敲低显著抑制增殖并促进凋亡。