一种由环吡咯-咪唑聚酰胺识别的 CAG/CTG 重复 DNA（5'-dWGCWGCW-3'）。
Strong and Specific Recognition of CAG/CTG Repeat DNA (5'-dWGCWGCW-3') by a Cyclic Pyrrole-Imidazole Polyamide.
机构信息
Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan.
Present address: Department of Genomic Neurology, Institute of Molecular Embryology and Genetics, Kumamoto University, Honjo, Chuo-ku, Kumamoto, 860-0811, Japan.
出版信息
Chembiochem. 2022 Jan 19;23(2):e202100533. doi: 10.1002/cbic.202100533. Epub 2021 Nov 18.
Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole-imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double-stranded DNA melting temperature (T ) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next-generation sequencing showed that the cPIP had high specificity for its target DNA sequence.
异常扩展的 CAG/CTG 重复 DNA 序列会导致多种神经退行性疾病，如亨廷顿病。在这里，我们合成了一种环状吡咯-咪唑聚酰胺（cPIP），它可以与 CAG/CTG DNA 序列的小沟结合。双链 DNA 熔点（T）和表面等离子体共振分析显示了 cPIP 的高结合亲和力。此外，下一代测序表明 cPIP 对其靶 DNA 序列具有高特异性。
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