共价小分子-RNA 复合物的形成能够实现小分子-RNA 相互作用的细胞分析。
Covalent small-molecule-RNA complex formation enables cellular profiling of small-molecule-RNA interactions.
机构信息
Department of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, 3A1, Jupiter, FL 33458 (USA).
出版信息
Angew Chem Int Ed Engl. 2013 Sep 16;52(38):10010-3. doi: 10.1002/anie.201301639. Epub 2013 Aug 1.
Abstract
Won't let you go! A strategy is described to design small molecules that react with their cellular RNA targets. This approach not only improves the activity of compounds targeting RNA in cell culture by a factor of about 2500 but also enables cell-wide profiling of its RNA targets.
摘要
不让你走！描述了一种设计小分子与细胞内 RNA 靶标反应的策略。这种方法不仅将化合物靶向细胞内 RNA 的活性提高了约 2500 倍，而且还能够对其 RNA 靶标进行全细胞分析。
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Covalent small-molecule-RNA complex formation enables cellular profiling of small-molecule-RNA interactions.共价小分子-RNA 复合物的形成能够实现小分子-RNA 相互作用的细胞分析。
Angew Chem Int Ed Engl. 2013 Sep 16;52(38):10010-3. doi: 10.1002/anie.201301639. Epub 2013 Aug 1.
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本文引用的文献
1
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Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1462-5. doi: 10.1002/anie.201206888. Epub 2012 Dec 20.
2
RNA splicing is responsive to MBNL1 dose.RNA 剪接对 MBNL1 剂量有反应。
PLoS One. 2012;7(11):e48825. doi: 10.1371/journal.pone.0048825. Epub 2012 Nov 15.
3
From dynamic combinatorial 'hit' to lead: in vitro and in vivo activity of compounds targeting the pathogenic RNAs that cause myotonic dystrophy.从动态组合的“命中”到先导物：针对导致肌营养不良症的致病 RNA 的化合物的体外和体内活性。
Nucleic Acids Res. 2012 Jul;40(13):6380-90. doi: 10.1093/nar/gks298. Epub 2012 Apr 6.
4
Rationally designed small molecules targeting the RNA that causes myotonic dystrophy type 1 are potently bioactive.针对导致 1 型肌强直性营养不良的 RNA 进行合理设计的小分子具有很强的生物活性。
ACS Chem Biol. 2012 May 18;7(5):856-62. doi: 10.1021/cb200408a. Epub 2012 Mar 5.
5
Design of a bioactive small molecule that targets the myotonic dystrophy type 1 RNA via an RNA motif-ligand database and chemical similarity searching.通过 RNA 基序-配体数据库和化学相似性搜索设计靶向肌强直性营养不良 1 型 RNA 的生物活性小分子。
J Am Chem Soc. 2012 Mar 14;134(10):4731-42. doi: 10.1021/ja210088v. Epub 2012 Mar 5.
6
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Anal Bioanal Chem. 2012 Feb;402(5):1889-98. doi: 10.1007/s00216-011-5604-0. Epub 2012 Jan 5.
7
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9
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J Biol Chem. 2011 Sep 30;286(39):34224-33. doi: 10.1074/jbc.M111.236547. Epub 2011 Aug 9.
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