Stefaniuk Catherine M, Schlegelmilch June, Meyerson Howard J, Harding Clifford V, Maitta Robert W
Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH, USA.
Case Western Reserve University School of Medicine, Cleveland, OH, USA.
J Thromb Thrombolysis. 2022 May;53(4):950-953. doi: 10.1007/s11239-021-02607-z. Epub 2021 Nov 19.
Over the last few years data from our group have indicated that α-synuclein is important in development of immune cells as well as potentially erythrocytes and platelets. The latter is important since this protein may work as negative regulator of granule release. Thus, we sought to begin to understand the structure of this protein in platelets. Flow cytometric analysis of this protein using region-specific (N-terminus, central region and C-terminus) monoclonal antibodies was performed. Antibody to the central region gave the strongest shift among all three antibodies, with the C-terminus having intermediate shift and N-terminus minimal shift. Western blotting using the same antibodies showed similar binding of all antibodies to α-synuclein. These results suggest a similar arrangement of this protein in platelets as seen in neurons. Future studies ought to look at the role that each protein region plays in platelets.
在过去几年里，我们团队的数据表明，α-突触核蛋白在免疫细胞以及可能的红细胞和血小板发育中起着重要作用。后者很重要，因为这种蛋白质可能作为颗粒释放的负调节因子发挥作用。因此，我们试图开始了解这种蛋白质在血小板中的结构。我们使用区域特异性（N端、中央区域和C端）单克隆抗体对这种蛋白质进行了流式细胞术分析。针对中央区域的抗体在所有三种抗体中产生的偏移最强，C端具有中等偏移，N端偏移最小。使用相同抗体进行的蛋白质印迹显示，所有抗体与α-突触核蛋白的结合相似。这些结果表明，这种蛋白质在血小板中的排列与在神经元中所见相似。未来的研究应该关注每个蛋白质区域在血小板中所起的作用。
