Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
Int J Biol Macromol. 2021 Dec 15;193(Pt B):2062-2069. doi: 10.1016/j.ijbiomac.2021.11.038. Epub 2021 Nov 16.
Cold causes oxidative stress in living organisms, mainly caused by the accumulation of reactive oxygen species (ROS). In the antioxidant defense systems, Glutathione S-transferases (GSTs) play a vital role in the regulation of detoxification and redox balance of ROS. In this study, the P. canaliculata GST gene family were characterized using a genome-wide search of the conserved domain. Phylogenetic tree and domain composition analysis revealed that 30 PcGSTs belong to seven classes, including five in MAPEG, two in Mu, nine in Omega, 11 in Sigma, one in Rho, Theta and Alpha class, respectively. RNA-seq analysis revealed that most PcGSTs localized in mitochondria highly expressed in hepatopancreas, and most PcGSTs localized in cytoplasm highly expressed in kidney. A total of 12 PcGST genes were significantly up-regulated and PcGST12 was significantly down-regulated after cold acclimation. Ten PcGSTs were identified as DEGs under cold stress after cold acclimation. qRT-PCR revealed that the expression level of five PcGST genes were significantly varied during the cold acclimation. The present study investigated the characterization of the P. canaliculata GST gene family, extending our understanding of GST mediated cold acclimation and cold stress-response mechanisms in this invasive snail.
冷胁迫会导致生物体内产生氧化应激，主要是由活性氧（ROS）的积累引起的。在抗氧化防御系统中，谷胱甘肽 S-转移酶（GSTs）在调节解毒和 ROS 的氧化还原平衡方面起着至关重要的作用。在这项研究中，通过对保守结构域的全基因组搜索，对 P. canaliculata GST 基因家族进行了特征描述。系统发生树和结构域组成分析表明，30 个 PcGSTs 属于七个类别，包括五个 MAPEG 家族、两个 Mu 家族、九个 Omega 家族、11 个 Sigma 家族、一个 Rho 家族、Theta 家族和 Alpha 家族。RNA-seq 分析表明，大多数 PcGSTs 定位于线粒体，在肝胰腺中高表达，而大多数 PcGSTs 定位于细胞质，在肾脏中高表达。在冷驯化后，共有 12 个 PcGST 基因显著上调，PcGST12 显著下调。10 个 PcGSTs 在冷驯化后的冷胁迫下被鉴定为差异表达基因。qRT-PCR 显示，在冷驯化过程中，五个 PcGST 基因的表达水平有显著差异。本研究对 P. canaliculata GST 基因家族进行了特征描述，扩展了我们对 GST 介导的冷驯化和入侵蜗牛冷应激反应机制的理解。
