Department of Clinical Laboratory, The Third People's Hospital of Nantong, Nantong, China.
Medical School of Nantong University, 117814Nantong University, Nantong, China.
Cancer Control. 2021 Jan-Dec;28:10732748211055681. doi: 10.1177/10732748211055681.
As a new class of non-coding RNAs, circRNAs have been recently reported to be involved in the tumorigenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a novel circRNA (hsa_circ_0013290) in hepatocellular carcinoma (HCC).
Relative hsa_circ_0013290 expression was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The subcellular location of hsa_circ_0013290 was performed by RNA subcellular isolation and fluorescence in situ hybridization (FISH) assays. The effect of hsa_circ_0013290 on proliferation was detected by Cell Counting Kit-8 (CCK-8) assays. The effect of hsa_circ_0013290 on cell cycle distribution and apoptosis was detected by flow cytometry. The invasion and migration abilities of hsa_circ_0013290 were detected by transwell assays.
Hsa_circ_0013290 is significantly upregulated in HCC cell lines and mainly located in cytoplasm of HCC cells. Hsa_circ_0013290 overexpression promotes cell invasion and migration and inhibits cell apoptosis. In contrast, hsa_circ_0013290 knockdown impedes cell invasion and migration and accelerates cell apoptosis. However, hsa_circ_0013290 did not affect cell proliferation.
Hsa_circ_0013290 is overexpressed in HCC cell lines and is mainly located in the cytoplasm of HCC cells. Hsa_circ_0013290 promotes cell invasion and migration, and inhibits cell apoptosis.
作为一类新的非编码 RNA，circRNAs 最近被报道参与了人类癌症的发生和发展。在本研究中，我们试图探索一种新型 circRNA（hsa_circ_0013290）在肝细胞癌（HCC）中的潜在功能。
通过定量逆转录聚合酶链反应（qRT-PCR）分析相对 hsa_circ_0013290 表达。通过 RNA 亚细胞分离和荧光原位杂交（FISH）实验检测 hsa_circ_0013290 的亚细胞定位。通过细胞计数试剂盒-8（CCK-8）实验检测 hsa_circ_0013290 对增殖的影响。通过流式细胞术检测 hsa_circ_0013290 对细胞周期分布和凋亡的影响。通过 Transwell 实验检测 hsa_circ_0013290 的侵袭和迁移能力。
hsa_circ_0013290 在 HCC 细胞系中显著上调，主要位于 HCC 细胞的细胞质中。hsa_circ_0013290 过表达促进细胞侵袭和迁移，抑制细胞凋亡。相反，hsa_circ_0013290 敲低则阻碍细胞侵袭和迁移，加速细胞凋亡。然而，hsa_circ_0013290 并不影响细胞增殖。
hsa_circ_0013290 在 HCC 细胞系中高表达，主要位于 HCC 细胞的细胞质中。hsa_circ_0013290 促进细胞侵袭和迁移，抑制细胞凋亡。
