将前列腺素转化为对-(9-蒽氧基)苯甲酰酯后，用高效液相色谱法进行检测。

Detection of prostaglandins by high-performance liquid chromatography after conversion to p-(9-anthroyloxy)phenacyl esters.

作者信息

Salari H, Yeung M, Douglas S, Morozowich W

机构信息

Department of Medicine, University of British Columbia, Vancouver General Hospital Research Institute, Canada.

出版信息

Anal Biochem. 1987 Aug 15;165(1):220-9. doi: 10.1016/0003-2697(87)90223-5.

DOI:10.1016/0003-2697(87)90223-5

PMID:3479910

Abstract

p-(9-Anthroyloxy)phenacyl bromide (panacyl bromide) undergoes rapid reaction with the carboxyl group of prostaglandins in the presence of N,N-diisopropylethylamine in acetonitrile-tetrahydrofuran (4:1). The resulting prostaglandin panacyl esters are strongly uv absorbing with a lambda max at 253 nm and an epsilon of 174,280 in acetonitrile. The lower limit of detection of prostaglandins was approximately 200 pg with uv detection (254 nm) and about 30 pg with fluorescent detection (exitation 253 and emission 445 nm) using normal-phase HPLC. The reactivity of panacyl bromide with 23 prostaglandins as well as prostaglandins released by human lung tissues was investigated.

摘要

对（9-蒽氧基）苯甲酰溴（泛酰溴）在乙腈-四氢呋喃（4:1）中，于N，N-二异丙基乙胺存在下，能与前列腺素的羧基迅速反应。生成的前列腺素泛酰酯具有强烈的紫外吸收，在乙腈中的最大吸收波长为253nm，摩尔吸光系数为174280。使用正相高效液相色谱法，采用紫外检测（254nm）时，前列腺素的检测下限约为200pg；采用荧光检测（激发波长253nm，发射波长445nm）时，检测下限约为30pg。研究了泛酰溴与23种前列腺素以及人肺组织释放的前列腺素的反应活性。