前列腺素15-脱氢酶体外孵育过程中形成的前列腺素的高效液相色谱分析。
High-performance liquid chromatographic analysis of prostaglandins formed during in vitro incubations with prostaglandin 15-dehydrogenase.
作者信息
Fitzpatrick F A
出版信息
J Pharm Sci. 1976 Nov;65(11):1609-13. doi: 10.1002/jps.2600651112.
Dinoprost, dinoprostone, and prostaglandin E1 were each separated from their major 15-keto metabolites by high-performance liquid chromatography on a microparticulate, bonded, reversed-phase column after conversion to their p-bromophenacyl esters. Detection and simultaneous quantitation of prostaglandins in 1.0 ml of a 5-muM solution is possible. The method was applied to monitor prostaglandins formed during in vitro incubations with prostaglandin 15-dehydrogenase from monkey lungs. The advantages of this technique for assessing enzyme purity and activity are discussed.
将地诺前列素、地诺前列酮和前列腺素E1转化为对溴苯甲酰酯后，在微粒键合反相柱上通过高效液相色谱法将它们与其主要的15-酮代谢物分离。可以检测并同时定量1.0 ml 5 μM溶液中的前列腺素。该方法用于监测在与猴肺前列腺素15-脱氢酶进行体外孵育过程中形成的前列腺素。讨论了该技术在评估酶纯度和活性方面的优势。
Zotero 插件