State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Harbin Institute of Technology, 73, Huanghe Rd, Nangang Dist, Harbin 150090, China.
J Hazard Mater. 2022 Feb 15;424(Pt D):127716. doi: 10.1016/j.jhazmat.2021.127716. Epub 2021 Nov 10.
Decabromodiphenyl ethane (DBDPE) can pose a potential toxic threat to human beings and the environment. P. ostreatus, as one of the typical white-rot fungi, can effectively degrade various refractory pollutants. The biodegradable characteristics of DBDPE by P. ostreatus, as well as the mechanisms, and toxicological response were investigated in this study. The removal rate reached 47.73% and 43.20%, respectively, for 5 and 20 mg/L DBDPE after 120-h degradation by P. ostreatus. As a coexisting substance, Pb could inhibit the biodegradation. It is found that both the intracellular enzyme (P450) and extracellular enzymes (manganese peroxidase (MnP), lignin peroxidase (LiP), and laccase (Lac)) played a very important role in the biodegradation of DBDPE, of which Lac dominated the degradation. The toxic response was monitored during the degradation. The activities of SOD and CAT were enhanced to eliminate excess ROS in P. ostreatus triggered by DBDPE. In addition, debromination, hydroxylation, and oxidation were inferred as the main degradation pathways preliminarily. The findings provide a theoretical basis for the application of microbial degradation of DBDPE contamination.
十溴二苯乙烷（DBDPE）可能对人类和环境构成潜在的有毒威胁。糙皮侧耳（P. ostreatus）作为一种典型的白腐真菌，能够有效降解各种难降解的污染物。本研究考察了糙皮侧耳对 DBDPE 的可生物降解特性、机制以及毒理响应。在 120 小时的降解后，5 和 20 mg/L 的 DBDPE 的去除率分别达到 47.73%和 43.20%。作为共存物质，Pb 会抑制生物降解。研究发现，细胞内酶（P450）和细胞外酶（锰过氧化物酶（MnP）、木质素过氧化物酶（LiP）和漆酶（Lac））在 DBDPE 的生物降解中都发挥了非常重要的作用，其中 Lac 主导了降解过程。在降解过程中监测了毒理响应。SOD 和 CAT 的活性增强，以消除 DBDPE 引发的糙皮侧耳中过量的 ROS。此外，推断脱溴、羟化和氧化是主要的降解途径。这些发现为微生物降解 DBDPE 污染的应用提供了理论依据。
