Efficient biodegradation of chlortetracycline in high concentration from strong-acidity pharmaceutical residue with degrading fungi.

Chlortetracycline (CTC) pharmaceutical residue with strong acidity and in high CTC concentration is a hazardous solid waste. There is a huge attention but few studies on whether and how the CTC raw residue (CRR) can be degraded in microbiological way. In this study, three self-screened fungi, LJ245, LJ302 and LJ318, were used and thoroughly investigated to remove CTC, strong acidity and biotoxicity in CRR. The result disclosed that the concentration of CTC decreased rapidly in the first seven days and declined slowly subsequently, and the decreasing curve was similar to "L" shape. the corresponding degradation ratios of three strains were 95.73%, 98.53% and 98.07%, respectively. Meanwhile, numerous intermediates in degradation appeared in early days and gradually reduced, and eventually disappeared once the degradation time was long enough, among which eleven intermediates from CTC were identified. Moreover, the strong acidity of CRR declined dramatically using this biological method along with the CTC being metabolized, the pH value increased from 2.30 to 8.32 in the first 7 days. The toxicity of CRR was significantly reduced by LJ302 with inhibition rate from 96.02% to no inhibition effect to Micrococcus luteus. Therefore, CTC, strong acidity and biotoxicity of CRR could be effectively removed simultaneously through a biodegradation process driven with proposed strains.