School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
School of Life Science, Beijing Institute of Technology, Beijing 100081, China; Université Paris Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy en Josas, France.
J Hazard Mater. 2022 Feb 15;424(Pt D):127671. doi: 10.1016/j.jhazmat.2021.127671. Epub 2021 Nov 3.
Chlortetracycline (CTC) pharmaceutical residue with strong acidity and in high CTC concentration is a hazardous solid waste. There is a huge attention but few studies on whether and how the CTC raw residue (CRR) can be degraded in microbiological way. In this study, three self-screened fungi, LJ245, LJ302 and LJ318, were used and thoroughly investigated to remove CTC, strong acidity and biotoxicity in CRR. The result disclosed that the concentration of CTC decreased rapidly in the first seven days and declined slowly subsequently, and the decreasing curve was similar to "L" shape. the corresponding degradation ratios of three strains were 95.73%, 98.53% and 98.07%, respectively. Meanwhile, numerous intermediates in degradation appeared in early days and gradually reduced, and eventually disappeared once the degradation time was long enough, among which eleven intermediates from CTC were identified. Moreover, the strong acidity of CRR declined dramatically using this biological method along with the CTC being metabolized, the pH value increased from 2.30 to 8.32 in the first 7 days. The toxicity of CRR was significantly reduced by LJ302 with inhibition rate from 96.02% to no inhibition effect to Micrococcus luteus. Therefore, CTC, strong acidity and biotoxicity of CRR could be effectively removed simultaneously through a biodegradation process driven with proposed strains.
金霉素（CTC）药物残留具有强酸性和高 CTC 浓度，是一种危险的固体废物。尽管人们对此高度关注，但关于 CTC 原始残留物（CRR）是否以及如何以微生物方式进行降解的研究却很少。在本研究中，使用了三种自行筛选的真菌 LJ245、LJ302 和 LJ318，对其去除 CRR 中的 CTC、强酸性和生物毒性进行了深入研究。结果表明，CTC 的浓度在前七天迅速下降，随后缓慢下降，下降曲线类似于“L”形。三种菌株的相应降解率分别为 95.73%、98.53%和 98.07%。同时，在降解早期出现了大量的中间产物，并逐渐减少，一旦降解时间足够长，最终会消失，其中有 11 种中间产物来自 CTC。此外，利用这种生物方法，随着 CTC 的代谢，CRR 的强酸性也会剧烈下降，pH 值从 2.30 增加到 7 天内的 8.32。CRR 的毒性通过 LJ302 显著降低，对微球菌的抑制率从 96.02%降低到无抑制作用。因此，通过所提出的菌株驱动的生物降解过程，可以有效去除 CRR 中的 CTC、强酸性和生物毒性。
