Ranchalis J E, Gentry L, Ogawa Y, Seyedin S M, McPherson J, Purchio A, Twardzik D R
Oncogen, Seattle, WA 98121.
Biochem Biophys Res Commun. 1987 Oct 29;148(2):783-9. doi: 10.1016/0006-291x(87)90944-2.
Two naturally occurring chrondogenesis inducing peptides have been purified to homogeneity from demineralized bovine bone. Cartilage-inducing factors A and B are the bone-derived equivalents of transforming growth factor-beta types I and II. Both peptides exhibit identical biological activities in chondrogenesis assays and stimulate anchorage independent cell growth. In this study we show that both bone-derived factors are potent (ng/ml) inhibitors of both DNA synthesis and the anchorage independent growth of a variety of human and non-human tumor cells. Unique in this study is also a comparison of the activities of these polypeptide growth factors with recombinant transforming growth factor type I expressed in mammalian cells.
两种天然存在的软骨生成诱导肽已从脱矿牛骨中纯化至同质。软骨诱导因子A和B分别相当于骨源性的转化生长因子-βⅠ型和Ⅱ型。这两种肽在软骨生成试验中表现出相同的生物学活性，并刺激非贴壁依赖性细胞生长。在本研究中，我们表明这两种骨源性因子均是多种人类和非人类肿瘤细胞DNA合成及非贴壁依赖性生长的强效（纳克/毫升）抑制剂。本研究的独特之处还在于将这些多肽生长因子的活性与在哺乳动物细胞中表达的重组转化生长因子Ⅰ型进行了比较。
