Wang Jin, Shi Xiaodi, Xiong Minghao, Tan Wen-Song, Cai Haibo
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
Cryobiology. 2022 Feb;104:47-55. doi: 10.1016/j.cryobiol.2021.11.004. Epub 2021 Nov 17.
The development of an effective cryopreservation method to achieve off-the-shelf and bioactive tissue-engineered constructs (TECs) is important to meet the requirements for clinical applications. The trehalose, a non-permeable cryoprotectant (CPA), has difficulty in penetrating the plasma membranes of mammalian cells and has only been used in combination with other cell penetrating CPA (such as DMSO) to cryopreserve mammalian cells. However, the inherent cytotoxicity of DMSO results in increasing risks with respect to cryopreserved cells. Therefore, in this study, permeable trehalose glycopolymers were synthesised for cryopreservation of TECs. The trehalose glycopolymers exhibited good ice inhibiting activities and biocompatibilities. Furthermore, the viability and function of TECs after cryopreservation with 5.0 wt% S2 were similar to those of the non-cryopreserved TECs. We developed an effective preservation strategy for the off-the-shelf availability of TECs.
开发一种有效的冷冻保存方法以实现即用型和生物活性组织工程构建体（TECs）对于满足临床应用需求至关重要。海藻糖是一种非渗透性冷冻保护剂（CPA），难以穿透哺乳动物细胞的质膜，仅与其他细胞穿透性CPA（如二甲基亚砜）联合使用来冷冻保存哺乳动物细胞。然而，二甲基亚砜固有的细胞毒性会增加冷冻保存细胞的风险。因此，在本研究中，合成了可渗透的海藻糖糖聚合物用于TECs的冷冻保存。海藻糖糖聚合物表现出良好的抑冰活性和生物相容性。此外，用5.0 wt% S2冷冻保存后TECs的活力和功能与未冷冻保存的TECs相似。我们为TECs的即用型可用性开发了一种有效的保存策略。
