通过对在含有石油工业采出水的培养基中培养的野油菜黄单胞菌进行LED照射，可提高黄原胶的产量和粘度。
Production and viscosity of Xanthan Gum are increased by LED irradiation of X. campestris cultivated in medium containing produced water of the oil industry.
作者信息
Crugeira Pedro J L, de Almeida Paulo F, Sampaio Igor C F, Soares Luiz G P, Moraga Amador David A, Samuel Ifor D W, Persheyev Saydulla, Silveira Landulfo, Pinheiro Antônio L B
机构信息
Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil; Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil.
Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil.
出版信息
J Photochem Photobiol B. 2022 Jan;226:112356. doi: 10.1016/j.jphotobiol.2021.112356. Epub 2021 Nov 12.
Oil recovery is a challenge and microbial enhanced oil recovery is an option. We theorized that the use of produced water (PW) with photo-stimulation could influence both production and viscosity of Xanthan gum. This study aimed at the evaluation of the effect of photo-stimulation by λ630 ± 1 ηm LED light on the biosynthesis of Xanthan gum produced by Xanthomonas campestris IBSBF 2103 strain reusing PW of the oil industry. We assessed the effect of photo-stimulation by LED light (λ630 nm) on the biosynthesis of Xanthan gum produced by X. campestris in medium containing produced water. Different energy densities applied during the microbial growth phase were tested. The highest production was achieved when using 12 J/cm LED light (p < 0.01). Three protocols were assessed: Non-irradiated (Control), Irradiation with LED light during the growth phase (LED) and Irradiation with LED light during both growth and production phases (LED ). Both the amount and viscosity of the xanthan gum was significantly higher (p < 0.01) in the group LED. The study showed that LED irradiation (λ630 ± 1 ηm) during both the growth and production phases of the biopolymer increased both the production and viscosity of Xanthan gum.
原油采收是一项挑战，而微生物强化采油是一种选择。我们推测，利用产出水（PW）并结合光刺激可能会影响黄原胶的产量和粘度。本研究旨在评估波长为630±1纳米的发光二极管（LED）光刺激对野油菜黄单胞菌IBSBF 2103菌株利用石油工业产出水生产黄原胶生物合成的影响。我们评估了LED光（波长630纳米）刺激对在含有产出水的培养基中野油菜黄单胞菌生产黄原胶生物合成的影响。测试了在微生物生长阶段施加的不同能量密度。使用12焦耳/平方厘米的LED光时产量最高（p<0.01）。评估了三种方案：未辐照（对照）、在生长阶段用LED光辐照（LED）以及在生长和生产阶段都用LED光辐照（LED ）。在LED组中，黄原胶的产量和粘度均显著更高（p<0.01）。研究表明，在生物聚合物的生长和生产阶段进行LED辐照（波长630±1纳米）可提高黄原胶的产量和粘度。
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