Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Sciences, Federal University of Bahia, Salvador, Brazil.
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Bragança, Portugal.
Biopolymers. 2022 Jun;113(6):e23488. doi: 10.1002/bip.23488. Epub 2022 Mar 26.
Produced water (PW) and crude glycerin (CG) are compounds overproduced by the oil and biodiesel industry and significant scientific efforts are being applied for properly recycling them. The aim of this research is to combine such industrial byproducts for sustaining the production of xanthan by Xanthomonas campestris. Xanthan yields and viscosity on distinct PW ratios (0, 10, 15, 25, 50, 100) and on 100% dialyzed PW (DPW) in shaker batch testing identified DPW treatment as the best approach for further bioreactor experiments. Such experiments showed a xanthan yield of 17.3 g/L within 54 h and a viscosity of 512 mPa s. Physical-chemical characterization (energy dispersive X-ray spectroscopy, scanning electron microscopy and Raman spectroscopy) showed similarities between the produced gum and the experimental control. This research shows a clear alternative for upcycling high salinity PW and CG for the generation of a valued bioproduct for the oil industry.
生产水 (PW) 和粗甘油 (CG) 是石油和生物柴油工业过度生产的化合物,目前正在进行大量科学研究以对其进行适当的回收利用。本研究旨在结合这些工业副产品,以维持黄单胞菌生产黄原胶。通过摇瓶分批试验,在不同 PW 比例(0、10、15、25、50、100)和 100%透析 PW(DPW)条件下对黄原胶产量和黏度进行了研究,结果表明 DPW 处理是进一步进行生物反应器试验的最佳方法。该试验在 54 小时内获得了 17.3 g/L 的黄原胶产量和 512 mPa·s 的黏度。物理化学特性分析(能量色散 X 射线光谱、扫描电子显微镜和拉曼光谱)表明,所生产的胶与实验对照品具有相似性。本研究为利用高盐度 PW 和 CG 生产高价值的石油工业生物制品提供了一种明确的升级回收方法。