Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal.
Instituto Gulbenkian de Ciências, Oeiras, Portugal.
Methods Mol Biol. 2022;2380:225-233. doi: 10.1007/978-1-0716-1736-6_19.
T follicular helper (Tfh) and T follicular regulatory (Tfr) cells are the two T cell subsets able to interact with B cells driving germinal center (GC) reactions. These T-B interactions are important for protective immune responses within secondary lymphoid tissue. However, the pathological emergence of ectopic lymphoid structures (ELS) that characterize several autoimmune diseases also involves Tfh and Tfr cells. ELS, often with ectopic GCs, can be identified through biopsies. Sjögren's syndrome (SS) is an example of an autoimmune disease where minor salivary gland (MSG) biopsies are often performed for diagnosis and where ELS can be found. Here, we describe a protocol to identify and isolate T follicular cells from MSGs by flow cytometry and immunohistochemistry.
滤泡辅助性 T 细胞（Tfh）和滤泡调节性 T 细胞（Tfr）是两种能够与 B 细胞相互作用驱动生发中心（GC）反应的 T 细胞亚群。这些 T-B 相互作用对于次级淋巴组织中的保护性免疫反应至关重要。然而，自身免疫性疾病特征性的异位淋巴样结构（ELS）的病理性出现也涉及 Tfh 和 Tfr 细胞。ELS 通常具有异位 GC，可以通过活检来识别。干燥综合征（SS）是一种自身免疫性疾病的例子，通常通过对唾液腺活检进行诊断，并且可以在其中发现 ELS。在这里，我们描述了一种通过流式细胞术和免疫组织化学从唾液腺中鉴定和分离 T 滤泡细胞的方案。
