The Francis Crick Institute, London, UK.
The National Heart and Lung Institute, Faculty of Medicine, Imperial College London, London, UK.
Methods Mol Biol. 2022;2380:235-254. doi: 10.1007/978-1-0716-1736-6_20.
The development of allergen-specific IgE is one of the hallmark symptoms of allergic diseases, including asthma. T follicular helper cells (TFH) are a subset of CD4 T cells that play a critical role in T-dependent antibody responses, including the generation of allergen-specific IgE. However, the role that TFH play in the pathogenesis of allergic disease is not completely understood especially as TFH produce IL-4 and IL-21 which are known to promote and prevent class switch recombination to IgE respectively. Here we describe methods of investigating TFH biology in the context of allergic airway inflammation, including how to set up mouse models of allergic airway disease, flow cytometric analysis of mouse TFH and detection of allergic-specific antibodies.
过敏原特异性 IgE 的产生是过敏疾病（包括哮喘）的标志性症状之一。滤泡辅助性 T 细胞（TFH）是 CD4 T 细胞的一个亚群，在 T 细胞依赖的抗体反应中发挥关键作用，包括过敏原特异性 IgE 的产生。然而，TFH 在过敏疾病发病机制中的作用尚不完全清楚，特别是因为 TFH 产生的 IL-4 和 IL-21 分别被认为促进和阻止向 IgE 的类别转换重组。在这里，我们描述了在过敏性气道炎症背景下研究 TFH 生物学的方法，包括如何建立过敏性气道疾病的小鼠模型、流式细胞术分析小鼠 TFH 和检测过敏特异性抗体。
