The University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia.
China-Australia Centre for Personalised Immunology, Renji Hospital, Shanghai Jiaotong University School of Medicine , Shanghai, China.
Methods Mol Biol. 2022;2380:255-265. doi: 10.1007/978-1-0716-1736-6_21.
T follicular helper (Tfh) cells are a subset of specialized CD4 T cell residing in B cell follicles and confer essential support for germinal center responses, which lead to the generation of long-lived humoral immunity. A great deal of evidence from the past 15 years indicate that excessive differentiation and dysregulated function of Tfh cells often promote autoimmunity by inducing autoantibody production. Interleukin-2 was identified as a major suppressor to inhibit Tfh differentiation. Therefore, IL-2 treatment was applied in suppressing Tfh function in mouse models and more recently in a clinical trial of patients with systemic lupus erythematosus. Here we describe a protocol for low-dose IL-2 treatment in a murine immunization model and on the assessment of the suppression of Tfh response using flow cytometry.
滤泡辅助性 T 细胞（Tfh）是一类存在于 B 细胞滤泡中的 CD4+T 细胞亚群，为生发中心反应提供必需的支持，进而产生长效体液免疫。过去 15 年的大量证据表明，Tfh 细胞的过度分化和功能失调常常通过诱导自身抗体产生而促进自身免疫。白细胞介素-2（IL-2）被鉴定为一种主要的抑制物，可抑制 Tfh 分化。因此，IL-2 治疗已被应用于抑制小鼠模型中的 Tfh 功能，并且最近在系统性红斑狼疮患者的临床试验中也得到了应用。在这里，我们描述了一种在小鼠免疫模型中进行低剂量 IL-2 治疗的方案，并介绍了使用流式细胞术评估 Tfh 反应抑制的方法。
