State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, China.
Sino-German Joint Research Institute, Nanchang University, Nanchang, China.
Phytochem Anal. 2022 Apr;33(3):441-451. doi: 10.1002/pca.3100. Epub 2021 Nov 21.
Plants containing aristolochic acid and its derivatives are nephrotoxic, mutagenic, and carcinogenic to humans; chronic diet poisoning caused by the aristolochic acid is the cause of endemic (Balkan) nephropathy and related cancers.
To develop a colloidal gold immunochromatographic test strip (ICS) based on the competitive format for the rapid detection of aristolochic acid A (AA-A) in herbal medicinal materials.
For the ICS based on gold nanoparticles (AuNPs), the antigen [AA-A-bovine serum albumin (BSA)], and goat anti-mouse IgG were drawn on the nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. Monoclonal antibody (MAb)-AuNP conjugates were sprayed onto the conjugate pad. The sensitivity of the ICS was 6 ng/mL, and the test was completed in 10 min. The analysis of AA-A in traditional Chinese medicine samples showed that the ICS results were in good agreement with those obtained by high-performance liquid chromatography methods.
These results demonstrated that the ICS test could be used as a reliable, rapid, cost-effective, and convenient qualitative tool for on-site screening techniques to detect AA-A in herbal medicinal materials without any special instrumentation.
含有马兜铃酸及其衍生物的植物对人类具有肾毒性、致突变性和致癌性；慢性饮食中毒由马兜铃酸引起，是地方性（巴尔干）肾病和相关癌症的病因。
开发基于竞争模式的胶体金免疫层析试验条（ICS），用于快速检测草药中的马兜铃酸 A（AA-A）。
对于基于金纳米粒子（AuNPs）的 ICS，抗原[AA-A-牛血清白蛋白（BSA）]和山羊抗小鼠 IgG 分别作为测试线（T 线）和控制线（C 线）绘制在硝酸纤维素膜上。单克隆抗体（MAb）-AuNP 缀合物被喷涂到结合垫上。ICS 的灵敏度为 6ng/mL，测试在 10 分钟内完成。对中药样品中的 AA-A 分析表明，ICS 结果与高效液相色谱法的结果非常吻合。
这些结果表明，ICS 试验可作为一种可靠、快速、具有成本效益且方便的现场筛选技术，用于检测草药中的 AA-A，而无需任何特殊仪器。
