Apicultural Research Institute, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Pestic Biochem Physiol. 2021 Nov;179:104975. doi: 10.1016/j.pestbp.2021.104975. Epub 2021 Sep 29.
Pesticides are one of the main causes of colony losses globally, posing a huge threat to the beekeeping industry. The fungicide carbendazim is commonly used on many crops worldwide, but the effects of fungicides on honey bees have received less attention than those of insecticides. Previous studies have shown that sublethal doses of carbendazim hinder growth and development and may destabilize and impede the development of honey bee colonies. The metabolome closely reflects brain activity at the functional level, allowing the effects of compounds such as fungicides to be investigated. Here, we established a model of carbendazim-treated honey bees, Apis mellifera, and used metabolomic approaches to better understand the effect of carbendazim on bee metabolic profiles. The results showed that 112 metabolites were significantly affected in carbendazim-treated bees compared to the control. Metabolites associated with energy and amino acid metabolism showed high abundance and were enriched for a wide range of pathways. In addition, the down-regulation of Aflatoxin B1exo-8,9-epoxide-GSH and glycerol diphosphate showed that carbenazim may affect the detoxification and immune system of honey bees. These results provide new insights into the interaction between fungicides and honey bees.
杀虫剂是全球蜂群损失的主要原因之一，对养蜂业构成了巨大威胁。杀菌剂多菌灵在世界范围内广泛用于许多作物，但杀菌剂对蜜蜂的影响比杀虫剂受到的关注要少。先前的研究表明，亚致死剂量的多菌灵会阻碍生长和发育，并可能破坏和阻碍蜜蜂群体的发展。代谢组学在功能水平上密切反映大脑活动，使我们能够研究杀菌剂等化合物的作用。在这里，我们建立了多菌灵处理的蜜蜂（Apis mellifera）模型，并使用代谢组学方法来更好地了解多菌灵对蜜蜂代谢谱的影响。结果表明，与对照组相比，多菌灵处理的蜜蜂中有 112 种代谢物受到显著影响。与能量和氨基酸代谢相关的代谢物表现出高丰度，并富集了广泛的途径。此外，Aflatoxin B1exo-8,9-epoxide-GSH 和甘油二磷酸的下调表明，多菌灵可能会影响蜜蜂的解毒和免疫系统。这些结果为杀菌剂与蜜蜂之间的相互作用提供了新的见解。
