Shinshu University, Department of Agricultural and Life Sciences, Faculty of Agriculture, 8304 Minamiminowa, Nagano 399-4598, Japan.
Shinshu University, Department of Agricultural and Life Sciences, Faculty of Agriculture, 8304 Minamiminowa, Nagano 399-4598, Japan.
Food Chem. 2022 Mar 30;373(Pt B):131617. doi: 10.1016/j.foodchem.2021.131617. Epub 2021 Nov 15.
Dried persimmons contain abundant insoluble tannins comprised of non-extractable proanthocyanidins (NEPAs). To determine the beneficial function of NEPAs, we studied the bile acid-binding ability of NEPAs using in vitro and in vivo experiments. For the in vitro evaluation, 70% (v/v) acetone-insoluble solids (AIS) with different NEPA levels was prepared from persimmon fruits during drying. AIS with higher NEPA levels displayed stronger bile acid-binding activity, and the highest NEPA levels can be obtained from mostly-dried fruits. The in vivo bile acid-excreting effect of dried persimmon powder (DP) and AIS was verified by feeding experiments using C57BL/6J mice. The average bile acid-excretion was in order of low-fat group (1.07 µmol/day) < high-fat (HF) group (3.03 µmol/day) < HF + AIS group (4.44 µmol/day) < HF + DP group (6.74 µmol/day). These results suggest that the bile acid-excreting function of the fruit is related to the presence of NEPAs, which are constitutive ingredients of dried persimmon.
柿饼含有丰富的不溶性单宁，由不可提取的原花青素（NEPAs）组成。为了确定 NEPAs 的有益功能，我们使用体外和体内实验研究了 NEPAs 的胆汁酸结合能力。对于体外评估，使用 70%（v/v）丙酮不溶性固体（AIS），从干燥过程中的柿子果实中制备出不同 NEPAs 水平的 AIS。具有更高 NEPAs 水平的 AIS 显示出更强的胆汁酸结合活性，并且可以从大部分干燥的果实中获得最高水平的 NEPAs。通过使用 C57BL/6J 小鼠进行喂养实验，验证了柿饼粉末（DP）和 AIS 的体内胆汁酸排泄作用。平均胆汁酸排泄的顺序为低脂组（1.07 µmol/天）<高脂肪（HF）组（3.03 µmol/天）<HF+AIS 组（4.44 µmol/天）<HF+DP 组（6.74 µmol/天）。这些结果表明，水果的胆汁酸排泄功能与 NEPAs 的存在有关，NEPAs 是柿饼的组成成分。
