Rearick J I, Hesterberg T W, Jetten A M
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
J Cell Physiol. 1987 Dec;133(3):573-8. doi: 10.1002/jcp.1041330320.
Epithelial cells of the airways can, under pathological conditions, undergo squamous metaplasia. The accumulation of cholesterol sulfate has recently been described as a new marker for squamous cell differentiation in rabbit tracheal epithelial cells. We now report that normal human bronchial epithelial cells in culture metabolically incorporated [35S]-sulfate and [3H]-mevalonate into material indistinguishable from cholesterol sulfate by the criteria of solubility in organic solvents, behavior on ion-exchange chromatography, susceptibility to solvolysis, and behavior on thin-layer chromatography before and after solvolysis. The accumulation of cholesterol [35S]-sulfate correlated well with squamous cell differentiation (as measured by cross-linked envelope formation), which occurred when the cells reached confluency. The increase in the level of cholesterol sulfate could be inhibited by the inclusion of retinoic acid in the cell-culture medium. The addition of phorbol-12-myristate-13-acetate or the presence of high Ca2+ concentration in the medium stimulated the accumulation of cholesterol sulfate. An increased activity of cholesterol sulfotransferase seems to account for the cholesterol sulfate accumulation. The original observation of cholesterol sulfate accumulation during squamous differentiation thus extends across species lines and strengthens the suggestion that the cholesterol sulfate may play an important role in this type of differentiation. Moreover, cholesterol sulfate provides a sensitive biochemical marker to study this pathway of differentiation of human bronchial epithelial cells.
在病理条件下，气道上皮细胞会发生鳞状化生。最近，硫酸胆固醇的积累被描述为兔气管上皮细胞鳞状细胞分化的一种新标志物。我们现在报告，培养的正常人支气管上皮细胞将[35S] - 硫酸盐和[3H] - 甲羟戊酸代谢掺入到通过在有机溶剂中的溶解度、离子交换色谱行为、对溶剂解的敏感性以及溶剂解前后的薄层色谱行为等标准与硫酸胆固醇无法区分的物质中。[35S] - 硫酸胆固醇的积累与鳞状细胞分化（通过交联包膜形成来衡量）密切相关，当细胞达到汇合时就会发生这种分化。细胞培养基中加入视黄酸可抑制硫酸胆固醇水平的升高。加入佛波醇 - 12 - 肉豆蔻酸 - 13 - 乙酸酯或培养基中高Ca2 + 浓度的存在会刺激硫酸胆固醇的积累。硫酸胆固醇转移酶活性的增加似乎是硫酸胆固醇积累的原因。因此，最初关于鳞状分化过程中硫酸胆固醇积累的观察结果跨越了物种界限，并强化了硫酸胆固醇可能在这种类型的分化中起重要作用的观点。此外，硫酸胆固醇为研究人类支气管上皮细胞的这种分化途径提供了一个敏感的生化标志物。
