CD8 T 细胞中 HLA-DR 表达水平与儿童急性传染性单核细胞增多症严重程度相关。

HLA-DR Expression Level in CD8 T Cells Correlates With the Severity of Children With Acute Infectious Mononucleosis.

机构信息

Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Department of Clinical Laboratory, First Affiliated Hospital of Nanchang University, Nanchang, China.

出版信息

Front Immunol. 2021 Nov 3;12:753290. doi: 10.3389/fimmu.2021.753290. eCollection 2021.

DOI:10.3389/fimmu.2021.753290

PMID:34804038

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8596082/

Abstract

BACKGROUND

This study aimed to assess the host immune signatures associated with EBV infection and its clinical value in indicating the severity of children with acute infectious mononucleosis (IM).

METHODS

Twenty-eight pediatric patients with IM aged 3-8 years were enrolled. The immune phenotypes and cytokine secretion capability of T cells were detected.

RESULTS

The percentages and absolute numbers of CD3 and CD8 T cells were significantly increased in IM patients compared with HCs. The percentages of Naïve CD4 and CD8 T cells were decreased but with increased percentages of memory CD4 and CD8 T subsets. Our results showed the upregulation of active marker HLA-DR, TCR-αβ, and inhibitory receptors PD-1, TIGIT in CD8 T cells from IM patients, which suggested that effective cytotoxic T cells were highly against EBV infection. However, EBV exposure impaired the cytokine (IFN-γ, IL-2, and TNF-α) secretion capability of CD4 and CD8 T cells after stimulation with PMA/ionomycin . Multivariate analysis revealed that the percentage of HLA-DR CD8 T cells was an independent prognostic marker for IM. The percentage of HLA-DR CD8 T cells was significantly correlated with high viral load and abnormal liver function results.

CONCLUSION

Robust expansion and upregulation of HLA-DR in CD8 T cells, accompanied with impaired cytokine secretion, were typical characteristics of children with acute IM. The percentage of HLA-DR CD8 T cells might be used as a prominent marker not only for the early diagnosis but also for indicating the severity of IM.

摘要

背景

本研究旨在评估与 EBV 感染相关的宿主免疫特征及其在指示儿童急性传染性单核细胞增多症 (IM) 严重程度方面的临床价值。

方法

纳入 28 名年龄为 3-8 岁的 IM 儿科患者。检测 T 细胞的免疫表型和细胞因子分泌能力。

结果

与 HC 相比，IM 患者的 CD3 和 CD8 T 细胞百分比和绝对数显著增加。幼稚 CD4 和 CD8 T 细胞的百分比降低，但记忆 CD4 和 CD8 T 亚群的百分比增加。我们的结果表明，IM 患者 CD8 T 细胞中活性标志物 HLA-DR、TCR-αβ 和抑制性受体 PD-1、TIGIT 的上调，这表明有效的细胞毒性 T 细胞高度针对 EBV 感染。然而，EBV 暴露后，PMA/离子霉素刺激的 CD4 和 CD8 T 细胞细胞因子（IFN-γ、IL-2 和 TNF-α）分泌能力受损。多变量分析显示 HLA-DR CD8 T 细胞的百分比是 IM 的独立预后标志物。HLA-DR CD8 T 细胞的百分比与高病毒载量和肝功能异常结果显著相关。