Amin Laila E, Salama Naglaa
Associate Professor of Oral Biology, Faculty of Dentistry, Mansoura University; Associate Professor of Oral Biology, Faculty of Dentistry, Horus University, Egypt.
Associate Professor of Oral Pathology, Faculty of Dentistry, Mansoura University; Associate Professor of Oral Pathology, Faculty of Dentistry, Horus University, Egypt.
Int J Dent. 2021 Oct 13;2021:8659010. doi: 10.1155/2021/8659010. eCollection 2021.
Osteoporosis is a progressive systematic skeletal illness characterized by low bone mineral density and susceptibility to fracture caused by bone resorption. . This study intended to evaluate the possible role of emdogain in combination with calcitonin on the healing of surgically induced mandibular defects performed on osteoporotic rats.
Forty healthy female white albino rats were included in this study and divided into four groups. In group I (negative control), 10 rats received a vehicle injection after which a unilateral mandibular defect was created in each rat of all groups. Three groups were subjected to induction of osteoporosis by subcutaneous injection of 0.1 mg/kg/day dexamethasone for 60 days. In group II, rats were kept without treatment. In group III, rats were treated with daily intramuscular injection of 2.5 IU/kg of synthetic salmon calcitonin. In group IV, rats were handled as group III, and the created cavity was filled with emdogain. Rats were euthanized at 2nd and 4th week postsurgically. Hematoxylin and eosin, Masson's trichrome, NF-B (nuclear factor of activated B cells), and immunohistochemical stains were used, followed by statistical analysis.
Group I showed normal stages of bone defects healing. Group II revealed the formation of granulation tissue with dilated blood vessels, while groups III and IV showed enhanced bone healing and proper collagen fibers. The percentage area of newly formed collagen fibers was significantly higher in group IV at 2nd week (13.96 ± 0.020%) and 4th week (16.95 ± 0.024%) than in group II (8.75 ± 0.015% and 10.29 ± 0.015%, respectively) and group III (12.93 ± 0.015% and 14.61 ± 0.021%, respectively), but was lower than that in group I (15.75 ± 0.015% and 17.49 ± 0.015%, respectively).
The local application of emdogain combined with systemically injected calcitonin improves bone healing in surgically induced bone defects in osteoporotic rats.
骨质疏松症是一种进行性系统性骨骼疾病，其特征为骨矿物质密度低以及因骨吸收而易于骨折。本研究旨在评估恩多盖因联合降钙素对骨质疏松大鼠手术诱导下颌骨缺损愈合的可能作用。
本研究纳入40只健康雌性白色白化大鼠，分为四组。在第一组（阴性对照组）中，10只大鼠接受赋形剂注射，之后所有组的每只大鼠均制造单侧下颌骨缺损。三组大鼠通过皮下注射0.1mg/kg/天的地塞米松诱导骨质疏松60天。第二组大鼠不进行治疗。第三组大鼠每日肌肉注射2.5IU/kg的合成鲑鱼降钙素。第四组大鼠的处理方式与第三组相同，且在制造的骨腔中填充恩多盖因。大鼠在术后第2周和第4周实施安乐死。使用苏木精-伊红染色、马松三色染色、NF-κB（活化B细胞核因子）染色及免疫组织化学染色，随后进行统计分析。
第一组显示骨缺损愈合的正常阶段。第二组可见伴有血管扩张的肉芽组织形成，而第三组和第四组显示骨愈合增强且胶原纤维正常。在第2周（13.96±0.020%）和第4周（16.95±0.024%）时，第四组新形成胶原纤维的面积百分比显著高于第二组（分别为8.75±0.015%和10.29±0.015%）及第三组（分别为12.93±0.015%和14.61±0.021%），但低于第一组（分别为15.75±0.015%和17.49±0.015%）。
恩多盖因局部应用联合全身注射降钙素可改善骨质疏松大鼠手术诱导骨缺损的骨愈合。
