Isola Gaetano, Polizzi Alessandro, Santonocito Simona, Alibrandi Angela, Williams Ray C
Department of General Surgery and Surgical-Medical Specialties, Unit of Oral Surgery and Periodontology, School of Dentistry, University of Catania, Catania, Italy.
Department of Economics, Unit of Statistical and Mathematical Sciences, University of Messina, Messina, Italy.
J Periodontol. 2022 Jan;93(1):135-145. doi: 10.1002/JPER.21-0049. Epub 2021 Jun 2.
Nod-like receptor family pyrin domain-containing protein-3 (NLRP3) complex inflammasome has potentially been shown to play an important role in the development of periodontitis and diabetes. The objective of this study was to analyze the association between serum and salivary NLRP3 concentrations in patients with periodontitis and type-II diabetes mellitus (DM) and to evaluate whether this association was influenced by potential confounders.
For the present study, a cohort of healthy controls (n = 32), and patients with periodontitis (n = 34), type-II DM (n = 33), and a combination of periodontitis + type-II DM (n = 34) were enrolled. Patients were characterized on the basis of their periodontal status and analyzed for demographic characteristics, serum mediators, and for serum and salivary concentrations of NLRP3. A uni- and multivariate model was established to analyze whether periodontitis, type-II DM, and CRP influenced serum and salivary NLRP3 concentrations.
In comparison to type-II DM patients and healthy controls, patients with periodontitis (serum, P = 0.003; saliva P = 0.012) and periodontitis + type-II DM (serum, P = 0.028; saliva, P = 0.003) had elevated serum and salivary NLRP3 concentrations. The multivariate regression model showed that periodontitis (P = 0.029) and HDL-cholesterol (P = 0.012) were significant predictors of serum NLRP3 concentrations whereas periodontitis (P = 0.036) and CRP (P = 0.012) were significant predictors of salivary NLRP3.
The results of the present study showed that periodontitis and periodontitis + type-II DM patients had higher serum and salivary NLRP3 concentrations in comparison to healthy controls and patients with type-II DM. Periodontitis was demonstrated to be a significant predictor of both serum and salivary NLRP3 concentrations.
含吡咯结构域的NOD样受体蛋白3（NLRP3）复合物炎性小体可能在牙周炎和糖尿病的发生发展中起重要作用。本研究的目的是分析牙周炎和2型糖尿病（DM）患者血清和唾液中NLRP3浓度之间的关联，并评估这种关联是否受到潜在混杂因素的影响。
在本研究中，纳入了一组健康对照者（n = 32）、牙周炎患者（n = 34）、2型糖尿病患者（n = 33）以及牙周炎合并2型糖尿病患者（n = 34）。根据患者的牙周状况进行特征描述，并分析其人口统计学特征、血清介质以及血清和唾液中NLRP3的浓度。建立单变量和多变量模型，以分析牙周炎、2型糖尿病和C反应蛋白（CRP）是否影响血清和唾液中NLRP3的浓度。
与2型糖尿病患者和健康对照者相比，牙周炎患者（血清，P = 0.003；唾液，P = 0.012）以及牙周炎合并2型糖尿病患者（血清，P = 0.028；唾液，P = 0.003）的血清和唾液中NLRP3浓度升高。多变量回归模型显示，牙周炎（P = 0.029）和高密度脂蛋白胆固醇（HDL - 胆固醇，P = 0.012）是血清NLRP3浓度的显著预测因素，而牙周炎（P = 0.036）和CRP（P = 0.012）是唾液NLRP3的显著预测因素。
本研究结果表明，与健康对照者和2型糖尿病患者相比，牙周炎患者以及牙周炎合并2型糖尿病患者的血清和唾液中NLRP3浓度更高。牙周炎被证明是血清和唾液中NLRP3浓度的重要预测因素。
