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2019冠状病毒病大流行期间的教学:分享从艾姆斯试验获得的结果和数据

Teaching during the COVID-19 Pandemic: Sharing Results and Data Obtained from the Ames Test.

作者信息

Blanco Valentina, Davyt Marcos, García-Alonso Javier, Piccini Claudia, Zunino Pablo, Rodríguez Eliana

机构信息

Unidad Académica de Laboratorios Prácticos, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.

Departamento de Ecología y Gestión Ambiental, Centro Universitario Regional Este - Maldonado, Universidad de la República, Maldonado, Uruguay.

出版信息

J Microbiol Biol Educ. 2021 Oct 29;22(3). doi: 10.1128/jmbe.00143-21. eCollection 2021 Dec.

DOI:10.1128/jmbe.00143-21
PMID:34804322
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8561835/
Abstract

We present a resource for instructors that contains results and data sets from the Ames test. Our aim is to share the results we have collected in previous semesters with other instructors, so they will be able to "conduct" the Ames test without the need to set foot in a laboratory classroom. Instructors will be able to use our online resource to perform the test remotely, as a supplement to their laboratory classroom, or even under hybrid circumstances. The coronavirus disease 2019 (COVID-19) pandemic brought many changes, including the way we, as instructors, were able to carry out our educational curricula, since access to laboratory classrooms was not always possible. While COVID-19 restrictions are still in place, and thus access to laboratory classrooms is limited or null, instructors can use our online resource, without the need to set foot in a laboratory classroom. When COVID-19 restrictions are lifted and access to laboratory classrooms is permitted, instructors can follow the procedures we describe and compare their results with ours, which appear in Results and Discussion, or use our data sets as take-home assignments for their students. In addition to its use in detecting the potential mutagenicity of different samples, we have found the Ames test to be extremely useful for developing problem-solving skills by means of exercises like the ones included in this resource. Furthermore, the potential of this test as a starting point for problem-based learning is remarkable. Some suggestions for its use in active learning settings are provided.

摘要

我们为教师提供了一个资源,其中包含艾姆斯试验的结果和数据集。我们的目的是与其他教师分享我们在前几个学期收集的结果,这样他们无需进入实验室教室就能“进行”艾姆斯试验。教师将能够使用我们的在线资源远程进行该试验,作为实验室课堂的补充,甚至在混合教学环境下使用。2019年冠状病毒病(COVID-19)大流行带来了许多变化,包括我们作为教师开展教学课程的方式,因为并非总能进入实验室教室。虽然COVID-19限制仍然存在,因此进入实验室教室受到限制或无法进入,但教师可以使用我们的在线资源,而无需进入实验室教室。当COVID-19限制解除且允许进入实验室教室时,教师可以按照我们描述的程序进行操作,并将他们的结果与我们在“结果与讨论”中呈现的结果进行比较,或者将我们的数据集用作给学生的课后作业。除了用于检测不同样品的潜在致突变性外,我们发现艾姆斯试验对于通过本资源中包含的练习等方式培养解决问题的能力非常有用。此外,该试验作为基于问题学习的起点的潜力非常显著。我们还提供了一些在主动学习环境中使用该试验的建议。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/77cde5b61e6c/jmbe.00143-21-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/1145889e9886/jmbe.00143-21-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/84a29a13709a/jmbe.00143-21-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/d60dd48a08bf/jmbe.00143-21-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/77cde5b61e6c/jmbe.00143-21-f004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/1145889e9886/jmbe.00143-21-f001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/84a29a13709a/jmbe.00143-21-f002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/d60dd48a08bf/jmbe.00143-21-f003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/210e/8561835/77cde5b61e6c/jmbe.00143-21-f004.jpg

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本文引用的文献

1
The test that changed the world: The Ames test and the regulation of chemicals.改变世界的试验:艾姆斯试验与化学品监管
Mutat Res Genet Toxicol Environ Mutagen. 2019 May;841:43-48. doi: 10.1016/j.mrgentox.2019.05.007. Epub 2019 May 15.
2
A Simple and Effective Method for Extracting Potential Mutagens from Sediment Samples in the Classroom Laboratory Setting.一种在课堂实验室环境中从沉积物样本中提取潜在诱变剂的简单有效方法。
J Microbiol Biol Educ. 2018 Apr 27;19(1). doi: 10.1128/jmbe.v19i1.1509. eCollection 2018.
3
The use of the ames test as a tool for addressing problem-based learning in the microbiology lab.
将艾姆斯试验用作微生物学实验室中解决基于问题的学习的工具。
J Microbiol Biol Educ. 2012 Dec 3;13(2):175-7. doi: 10.1128/jmbe.v13i2.421. Print 2012.
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Environ Health Perspect. 2010 Nov;118(11):1515-22. doi: 10.1289/ehp.1002336.
5
Reinventing the ames test as a quantitative lab that connects classical and molecular genetics.将艾姆斯试验重新打造为一个连接经典遗传学与分子遗传学的定量实验室。
Genetics. 2009 Jan;181(1):23-31. doi: 10.1534/genetics.108.095588. Epub 2008 Nov 17.
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The Ames Salmonella/microsome mutagenicity assay.艾姆斯沙门氏菌/微粒体诱变性试验。
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Recommendations for the performance of bacterial mutation assays.细菌突变试验的操作建议。
Mutat Res. 1994 Jun;312(3):217-33. doi: 10.1016/0165-1161(94)90037-x.
8
Revised methods for the Salmonella mutagenicity test.沙门氏菌致突变性试验的修订方法。
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