Maron D M, Ames B N
Mutat Res. 1983 May;113(3-4):173-215. doi: 10.1016/0165-1161(83)90010-9.
The methods for detecting carcinogens and mutagens with the Salmonella mutagenicity test were described previously (Ames et al., 1975b). The present paper is a revision of the methods. Two new tester strains, a frameshift strain (TA97) and a strain carrying an ochre mutation on a multicopy plasmid (TA102), are added to the standard tester set. TA97 replaces TA1537. TA1535 and TA1538 are removed from the recommended set but can be retained at the option of the investigator. TA98 and TA100 are retained. We discuss other special purpose strains and present some minor changes in procedure, principally in the growth, storage, and preservation of the tester strains. Two substitutions are made in diagnostic mutagens to eliminate MNNG and 9-aminoacridine. Some test modifications are discussed.
利用沙门氏菌致突变性试验检测致癌物和诱变剂的方法先前已有描述（艾姆斯等人，1975b）。本文是对这些方法的修订。标准测试菌株组中新增了两种新的测试菌株，一种移码突变菌株（TA97）和一种在多拷贝质粒上携带赭石突变的菌株（TA102）。TA97取代了TA1537。TA1535和TA1538从推荐菌株组中移除，但研究人员可根据需要保留。TA98和TA100保留。我们讨论了其他特殊用途菌株，并介绍了一些程序上的小变化，主要涉及测试菌株的培养、储存和保存。诊断诱变剂进行了两项替换，以去除MNNG和9-氨基吖啶。还讨论了一些测试修改。
