Naghizadeh Mehrdad, Mirshekar Mohammad Ali, Montazerifar Farzaneh, Saadat Saeideh, Shamsi Koushki Ali, Jafari Maskouni Saber, Afsharfar Maryam, Arabmoazzen Saiedeh
Student Research Committee, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Food Sciences and Nutrition, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Avicenna J Phytomed. 2021 Nov-Dec;11(6):599-609. doi: 10.22038/AJP.2021.18526.
Quercetin is one of the most popular flavonoid with protective effects against neural damages in Parkinson's disease (PD). We assessed the effect of quercetin administration on memory and motor function, hippocampal oxidative stress and brain-derived neurotrophic factor (BDNF) level in a 6-OHDA-induced Parkinson's rat model.
The animals were divided into the following five groups (n=8): control, sham-surgery (sham), lesion (PD), and lesion animals treated with quercetin at doses of 10 (Q10) and 25 (Q25) mg/kg. For induction of a model of PD, 6-OHDA was injected into the striatum of rats. The effects of quercetin were investigated on spatial memory, hippocampal BDNF and malondialdehyde (MDA) levels, and total antioxidant capacity (TAC). Spatial memory was assessed by Morris water maze test, and the neuronal firing frequency in hippocampal dentate gyrus (HDG) was evaluated by single-unit recordings.
Mean path length and latency time, rotational behavior and hippocampal MDA concentration were significantly increased, while time spent in the goal quadrant, swimming speed, spike rate, and hippocampal levels of TAC and BDNF were significantly decreased in the PD group compared to the sham group (p<0.01 to p<0.001). Quercetin treatment significantly enhanced time spent in goal quadrant (p<0.05), swimming speed (p<0.001) and spike rate (p<0.01), improved hippocampal TAC (p<0.05 to p<0.001) and BDNF (p<0.01 to p<0.001) level, and decreased mean path length (p<0.001), latency time (p<0.05 to p<0.001), rotational behavior and hippocampal MDA concentration (p<0.05).
The cognitive-enhancing effect of quercetin might be due to its antioxidant effects in the hippocampus.
槲皮素是最常见的黄酮类化合物之一，对帕金森病（PD）的神经损伤具有保护作用。我们评估了在6-羟基多巴胺（6-OHDA）诱导的帕金森病大鼠模型中，给予槲皮素对记忆和运动功能、海马氧化应激及脑源性神经营养因子（BDNF）水平的影响。
将动物分为以下五组（n = 8）：对照组、假手术组（假手术）、损伤组（PD）以及分别用10（Q10）和25（Q25）mg/kg剂量槲皮素处理的损伤动物组。为诱导PD模型，将6-OHDA注入大鼠纹状体。研究槲皮素对空间记忆、海马BDNF和丙二醛（MDA）水平以及总抗氧化能力（TAC）的影响。通过莫里斯水迷宫试验评估空间记忆，通过单单位记录评估海马齿状回（HDG）的神经元放电频率。
与假手术组相比，PD组的平均路径长度和潜伏期时间、旋转行为及海马MDA浓度显著增加，而在目标象限的停留时间、游泳速度、放电率以及海马TAC和BDNF水平显著降低（p < 0.01至p < 0.001）。槲皮素治疗显著增加了在目标象限的停留时间（p < 0.05）、游泳速度（p < 0.001）和放电率（p < 0.01），改善了海马TAC（p < 0.05至p < 0.001）和BDNF（p < 0.01至p < 0.001）水平，并降低了平均路径长度（p < 0.001）、潜伏期时间（p < 0.05至p < 0.001）、旋转行为及海马MDA浓度（p < 0.05）。
槲皮素的认知增强作用可能归因于其在海马中的抗氧化作用。
