通过细胞外通量分析研究细胞代谢的 K 敏感性。

Gottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Neue Stiftingtalstraße 6/6, 8010 Graz, Austria.

Department of Pharmacology, Toxicology and Clinical Pharmacy, Institute of Pharmacy, University of Tuebingen, Auf der Morgenstelle 8, 72076 Tuebingen, Germany.

STAR Protoc. 2021 Nov 5;2(4):100876. doi: 10.1016/j.xpro.2021.100876. eCollection 2021 Dec 17.

We have recently demonstrated that the activity of hexokinase 2 is dependent on the intracellular potassium ion (K) concentration ([K]). To analyze the K dependency of the cell metabolism in cell populations, we used an extracellular flux analyzer to assess oxygen consumption and acidification rates as well-established measures of oxidative- and glycolytic metabolic activities. This protocol describes in detail how a potential K sensitivity of the cell metabolism can be elucidated by extracellular flux analysis. For complete details on the use and execution of this protocol, please refer to Bischof et al. (2021).

我们最近证明，己糖激酶 2 的活性依赖于细胞内钾离子（K）浓度([K])。为了分析细胞群体中细胞代谢的 K 依赖性，我们使用细胞外通量分析仪来评估氧消耗和酸化率，这是氧化和糖酵解代谢活性的既定测量方法。本协议详细描述了如何通过细胞外通量分析阐明细胞代谢的潜在 K 敏感性。有关该协议的使用和执行的完整详细信息，请参阅 Bischof 等人。（2021 年）。