Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Molecular Engineering & Sciences Institute, University of Washington, Seattle, WA 98105, USA.
STAR Protoc. 2021 Nov 5;2(4):100938. doi: 10.1016/j.xpro.2021.100938. eCollection 2021 Dec 17.
Gastrointestinal motility is regulated by a variety of environmental factors including gut microbes and metabolites. The ability to interrogate mouse models of gut motility has enabled elucidation of these relationships. Here we describe integration of the red carmine dye and fluorescence isothiocyanate-dextran marker-based assays for characterizing gut transit with spatial resolution, along with an optional intracolonic infusion protocol for studying the effects of metabolites on colonic transit. These protocols can be adapted for use in gnotobiotic and conventional mouse models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
胃肠道蠕动受多种环境因素的调节，包括肠道微生物和代谢物。能够研究肠道蠕动的小鼠模型使这些关系得以阐明。在这里，我们描述了整合红色胭脂红染料和荧光异硫氰酸荧光素标记的葡聚糖标记物检测法，以具有空间分辨率的方式来描述肠道通过的特征，以及一个可选的用于研究代谢物对结肠通过的影响的腔内灌注方案。这些方案可以适应于无菌和常规的小鼠模型。有关此方案的使用和实施的完整详细信息，请参见 Li 等人。（2021）。
