Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Universidad Santo Tomás, Osorno, Chile; Magister en Ciencias Mención Microbiología, Universidad Austral de Chile, Valdivia, Chile.
Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Centro FONDAP-IDEAL, Universidad Austral de Chile, Valdivia, Chile.
Fish Shellfish Immunol. 2022 Jan;120:695-705. doi: 10.1016/j.fsi.2021.11.026. Epub 2021 Nov 19.
The brain's immune system is selective and hermetic in most species, including fish, favoring immune responses mediated by soluble immunomodulatory factors such as serotonin and the availability of nutrients against infectious processes. Francisella noatunensis coexist with fish such as Eleginops maclovinus, which raises questions about the susceptibility and immune response of the brain of E. maclovinus against Francisella. In this study, we inoculated fish with different doses of Francisella and took samples for 28 days. We detected bacteria in the brain of fish injected with a high concentration of Francisella at all time points. qPCR analysis of immune genes indicated a response mainly in the medium-dose and early expression of genes involved in iron metabolism. Finally, brain serotonin levels were higher than in uninfected fish in all conditions, suggesting possible immunomodulatory participation in an infectious process.
在包括鱼类在内的大多数物种中，大脑的免疫系统是具有选择性和密闭性的，它有利于通过可溶性免疫调节因子（如血清素）和营养物质的可用性来介导免疫反应，以对抗感染过程。弗朗西斯氏菌与 Eleginops maclovinus 等鱼类共存，这引发了对 Eleginops maclovinus 大脑对弗朗西斯氏菌易感性和免疫反应的疑问。在这项研究中，我们用不同剂量的弗朗西斯氏菌感染鱼类，并在 28 天内取样。我们在所有时间点都检测到了高浓度弗朗西斯氏菌注射鱼类的大脑中的细菌。免疫基因的 qPCR 分析表明，在中剂量和早期，与铁代谢相关的基因表达主要受到了影响。最后，在所有情况下，感染鱼的大脑中的血清素水平均高于未感染的鱼，这表明其可能参与了免疫调节的感染过程。
