Naughton B A, Preti R A, Naughton G K
Medical Laboratory Sciences Dept., Hunter College School of Health Sciences, New York, New York 10010.
J Med. 1987;18(3-4):219-50.
Rodent hematopoietic cells have been perpetuated in long-term bone marrow culture (LTBMC), provided that they were plated onto a pre-established layer of bone marrow stromal cells (fibroblasts, reticular cells, adipocytes, macrophages, etc.). This monolayer-type system supports the self-renewal of murine pluripotent stem cells and produces substantial numbers of progenitors and mature cells of the myeloid lineage. In an effort to increase the growth potential of cells in LTBMC, an adherent matrix of stromal cells was established on a pretreated nylon screen template. Subsequent seeding of hematopoietic cells onto this matrix occurred both on the surface of the adherent layer and in the interstices formed by the developing stromal cells. A three dimensional growth pattern of hematopoietic colonies and clusters was observed. Mature cells and late stage precursors of the myeloid and erythroid series were observed in the non-adherent layer for the duration of the experiment (39 weeks). Cells similar in appearance to small lymphocytes were also seen in both the non-adherent and adherent layers. Cells associated with the adherent layer of this LTBMC system displayed the ability to reconstitute hematopoiesis in the irradiated host. It is suggested that because of its three-dimensional nature the nylon mesh LTBMC system possesses a greater hematologic potential per unit area than monolayer-type LTBMC.
啮齿动物造血细胞已在长期骨髓培养（LTBMC）中得以维持，前提是将它们接种到预先建立的骨髓基质细胞层（成纤维细胞、网状细胞、脂肪细胞、巨噬细胞等）上。这种单层系统支持小鼠多能干细胞的自我更新，并产生大量髓系谱系的祖细胞和成熟细胞。为了提高LTBMC中细胞的生长潜力，在预处理的尼龙筛模板上建立了基质细胞的附着基质。随后将造血细胞接种到该基质上，既接种在附着层表面，也接种在发育中的基质细胞形成的间隙中。观察到造血集落和簇的三维生长模式。在实验期间（39周），在非附着层中观察到了髓系和红系系列的成熟细胞和晚期前体细胞。在非附着层和附着层中还可见到外观与小淋巴细胞相似的细胞。与该LTBMC系统附着层相关的细胞显示出在受辐照宿主中重建造血的能力。有人提出，由于其三维性质，尼龙网LTBMC系统每单位面积具有比单层型LTBMC更大的血液学潜力。
