Fleming G H, Boynton J E, Gillham N W
Department of Botany, Duke University, Durham, NC 27706.
Mol Gen Genet. 1987 Dec;210(3):419-28. doi: 10.1007/BF00327192.
In vitro protein synthesis was used to characterize the antibiotic sensitivity of cytoplasmic ribosomes from wild-type and antibiotic-resistant strains of Chlamydomonas reinhardtii. Cytoplasmic ribosomes from two cycloheximide-resistant mutants, act-1 and act-2, were resistant to the antibiotic in vitro. The alteration effected by the act-1 mutation, which was dominant in diploids, was localized to the large subunit of the cytoplasmic ribosomes, but no ribosomal protein alterations were detected using two-dimensional gel electrophoresis. The act-2 mutation, which was semidominant in diploids, was frequently associated with a charge alteration in the large subunit ribosomal protein (r-protein) cyL38 that segregated independently from the antibiotic-resistant phenotype in crosses.
利用体外蛋白质合成来表征莱茵衣藻野生型和抗生素抗性菌株的细胞质核糖体对抗生素的敏感性。来自两个抗放线菌酮突变体act-1和act-2的细胞质核糖体在体外对抗生素具有抗性。act-1突变在二倍体中呈显性，其引起的变化定位于细胞质核糖体的大亚基，但二维凝胶电泳未检测到核糖体蛋白的变化。act-2突变在二倍体中呈半显性，常与大亚基核糖体蛋白（r蛋白）cyL38的电荷变化相关，在杂交中该电荷变化与抗生素抗性表型独立分离。
