Dequard M, Couderc J L, Legrain P, Belcour L, Picard-Bennoun M
Biochem Genet. 1980 Apr;18(3-4):263-80. doi: 10.1007/BF00484241.
It has recently been shown that paromomycin, an antibiotic of the aminoglycoside family, is also active on eukaryotic cytoplasmic ribosomes. In the fungus Podospora anserina, genetic analysis of ten mutants resistant to high doses of paromomycin shows that this resistance is caused by mutations in two different nuclear genes. These mutants display pleiotropic phenotypes (cold sensitivity, mycelium and spore appearance and coloration, cross-resistance to other antibiotics). Double mutants are either lethal or very altered and unstable. Moreover, the cytochrome spectra of these mutants seem to indicate that cytoplasmic protein synthesis is affected. The mutants also display a slight suppressor effect. We can therefore assume that these mutations affect cytoplasmic ribosomes.
最近研究表明，氨基糖苷类抗生素巴龙霉素对真核细胞质核糖体也有活性。在鹅掌柄孢壳菌中，对高剂量巴龙霉素耐药的10个突变体的遗传分析表明，这种耐药性是由两个不同核基因的突变引起的。这些突变体表现出多效性表型（冷敏感性、菌丝体和孢子外观及颜色、对其他抗生素的交叉耐药性）。双突变体要么致死，要么发生很大改变且不稳定。此外，这些突变体的细胞色素光谱似乎表明细胞质蛋白质合成受到影响。这些突变体还表现出轻微的抑制作用。因此我们可以假定这些突变影响细胞质核糖体。
