Department of Pediatric and Preventive Dentistry, Stes's Sinhgad Dental College and Hospital, Pune, Maharashtra, India.
Department of Orthodontics and Dentofacial Orthopedics, Stes's Sinhgad Dental College and Hospital, Pune, Maharashtra, India.
J Indian Soc Pedod Prev Dent. 2021 Jul-Sep;39(3):279-283. doi: 10.4103/jisppd.jisppd_519_20.
Probiotics compete with the cariogenic oral bacteria like Streptococcus mutans (S. mutans) for carbohydrates resulting in substrate depletion and reduction in the number of S. mutans.
The evaluation of topical effect of short-term application of Probiotic formulation on S. mutans counts in plaque of 7-12 years old children with decayed, missing, filled teeth (dmft/DMFT) score 2-5.
A registered randomized clinical trial (Clinical Trials Registry of India/ICMR - CTRI/2019/09/021205) was conducted on 7-12 years medium risk 10 children with dmft/DMFT score 2-5, where an innovative probiotic formulation (prepared from capsule) was applied for 6 consecutive days, on teeth and pre/post plaque samples were evaluated for S. mutans count on mitis salivarius agar.
There was statistically significant and drastic reduction from 608,200 to 6600 (>90%) in the S. mutans count in plaque after the application of probiotic solution for a short period of time (6 days).
Short-term application of probiotics can be used as an alternate topical preventive therapy to reduce the count of S. mutans effectively in oral cavity/plaque. Hence, probiotic can be used as topical caries preventive agent.
益生菌与致龋口腔细菌（如变形链球菌，S. mutans）竞争碳水化合物，导致基质消耗和 S. mutans 数量减少。
评估短期应用益生菌配方对 7-12 岁龋失补牙面数（dmft/DMFT）为 2-5 的儿童牙菌斑中 S. mutans 计数的局部作用。
在印度临床试验注册中心（ICMR - CTRI/2019/09/021205）注册的一项随机临床试验中，对 7-12 岁中危 10 名 dmft/DMFT 评分 2-5 的儿童进行了研究，在这 10 名儿童中，应用了一种创新的益生菌配方（胶囊制备），连续 6 天应用于牙齿上，评估治疗前后牙菌斑中 S. mutans 计数，采用含唾液链球菌的琼脂培养基。
在短时间（6 天）内应用益生菌溶液后，牙菌斑中 S. mutans 的计数从 608200 减少到 6600（>90%），差异具有统计学意义。
短期应用益生菌可作为一种替代局部预防性治疗方法，有效降低口腔/牙菌斑中 S. mutans 的数量。因此，益生菌可用作局部防龋剂。
