Department of Food Science and Engineering, School of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, 315832, People's Republic of China.
Key Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315832, People's Republic of China.
Int Microbiol. 2022 Aug;25(3):417-426. doi: 10.1007/s10123-021-00217-6. Epub 2021 Nov 22.
Issatchenkia orientalis (I. orientalis) is tolerant to various environmental stresses especially acetic acid stress in wine making. However, limited literature is available on the transcriptome profile of I. orientalis under acetic acid stress. RNA-sequence was used to investigate the metabolic changes due to underlying I. orientalis 166 (Io 166) tolerant to acetic acid. Transcriptomic analyses showed that genes involved in ergosterol biosynthesis are differentially expressed under acetic acid stress. Genes associated with ribosome function were downregulated, while energy metabolism-related genes were upregulated. Moreover, Hsp70/Hsp90 and related molecular chaperones were upregulated to recognize and degrade misfolded proteins. Compared to Saccharomyces cerevisiae, transcriptomic changes of Io 166 showed many similarities under acetic acid stress. There were significant upregulation of genes in ergosterol biosynthesis and for the application of wine production.
东方伊萨酵母（I. orientalis）能够耐受各种环境压力，特别是在酿酒过程中耐受乙酸胁迫。然而，目前关于乙酸胁迫下东方伊萨酵母转录组谱的文献有限。本研究采用 RNA 测序技术研究了东方伊萨酵母 166（Io 166）耐受乙酸的代谢变化。转录组分析表明，在乙酸胁迫下，与麦角固醇生物合成相关的基因表达存在差异。与核糖体功能相关的基因下调，而与能量代谢相关的基因上调。此外，Hsp70/Hsp90 及其相关分子伴侣上调，以识别和降解错误折叠的蛋白质。与酿酒酵母相比，Io 166 在乙酸胁迫下的转录组变化表现出许多相似性。在麦角固醇生物合成和葡萄酒生产应用方面，基因的显著上调。
