评估伴有鼻息肉的慢性鼻-鼻窦炎中 MMP-12 的表达。
Evaluation of MMP-12 expression in chronic rhinosinusitis with nasal polyposis.
机构信息
Department of Otorhinolaryngology, University Hospital of Patras, Patras, Greece.
Department of Biochemistry, School of Medicine, University of Patras, Patras, Greece.
出版信息
Rhinology. 2022 Feb 1;60(1):39-46. doi: 10.4193/Rhin21.320.
BACKGROUND
The purpose of this study was to evaluate the expression of MMP-12 in patients with chronic rhinosinusitis with polyps (CRSwNP).
METHODOLOGY
Tissue samples from 37 patients with CRSwNP undergoing functional endoscopic sinus surgery and healthy mucosa specimens from 12 healthy controls were obtained intraoperatively. The mRNA and protein expression levels of MMP-12 were quantified by real-time polymerase chain reaction and Western blotting, respectively.
RESULTS
mRNA levels of MMP-12 were significantly elevated in the CRSwNP tissue samples compared to those in control ones. The protein levels of MMP-12 showed a trend of increasing but with no statistical significance.
CONCLUSIONS
Elevation of MMP-12 in patients with CRSwNP suggests its potential implication in the pathogenesis of the disease. The difference in the expression profile observed between mRNA and protein levels could be due to post-translational gene expression regulation. Our findings provide evidence that MMP-12 along with other MMPs may serve as a biomarker and therapeutic target in the management of the disease.
背景
本研究旨在评估基质金属蛋白酶-12（MMP-12）在慢性鼻息肉鼻窦炎（CRSwNP）患者中的表达。
方法
术中获取 37 例接受功能性内镜鼻窦手术的 CRSwNP 患者和 12 例健康对照者的组织样本。通过实时聚合酶链反应和 Western 印迹分别定量 MMP-12 的 mRNA 和蛋白表达水平。
结果
CRSwNP 组织样本中的 MMP-12 mRNA 水平明显高于对照组。MMP-12 蛋白水平呈升高趋势，但无统计学意义。
结论
CRSwNP 患者 MMP-12 的升高提示其可能参与疾病的发病机制。mRNA 和蛋白水平之间观察到的表达谱差异可能是由于翻译后基因表达调控所致。我们的研究结果表明，MMP-12 可能与其他 MMP 一起作为疾病管理的生物标志物和治疗靶点。
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