School of Chemistry, Xi'an Jiaotong University, Xi'an 710049, China.
Mikrochim Acta. 2021 Nov 23;188(12):427. doi: 10.1007/s00604-021-05096-2.
A chemiluminescence (CL) sensor was constructed for the one-step determination of glucose. Glucose oxidase (GOx) was successfully encapsulated into Zn-doped zeolitic imidazolate framework-67 (Zn-ZIF-67) via a simple one-pot strategy. The as-prepared GOx@Zn-ZIF-67 nanocomposite can trigger cascade reactions of glucose oxidation to generate HO and HO-mediated luminol reaction to give an intense CL emission. The sensor responds linearly to glucose in the 20.0-400.0 μmol·L range with a limit of detection (LOD) of 4.7 μmol·L. Eleven replicated measurements of 200.0 μmol·L glucose solution gives a relative standard deviation (RSD) of 1.7%. The sensor exhibits good selectivity and stability and was successfully applied to the determination of glucose in real human serum samples. Schematic representation of one-step determination of serum glucose with GOx@Zn-ZIF-67 nanocomposite triggering cascade reactions between luminol and glucose.
构建了一种用于一步测定葡萄糖的化学发光（CL）传感器。通过简单的一锅法，成功地将葡萄糖氧化酶（GOx）封装到锌掺杂沸石咪唑酯骨架-67（Zn-ZIF-67）中。所制备的 GOx@Zn-ZIF-67 纳米复合材料可以引发葡萄糖氧化的级联反应，生成 HO 和 HO 介导的鲁米诺反应，从而产生强烈的 CL 发射。该传感器对 20.0-400.0 μmol·L 范围内的葡萄糖呈线性响应，检测限（LOD）为 4.7 μmol·L。200.0 μmol·L 葡萄糖溶液的 11 次重复测量的相对标准偏差（RSD）为 1.7%。该传感器表现出良好的选择性和稳定性，并成功应用于实际人血清样品中葡萄糖的测定。GOx@Zn-ZIF-67 纳米复合材料触发鲁米诺和葡萄糖之间级联反应一步测定血清葡萄糖的示意图。
