College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China; NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China.
NHC Key Laboratory of Parasite and Vector Biology, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Tropical Diseases, Shanghai, 200025, China.
Mol Biochem Parasitol. 2022 Jan;247:111431. doi: 10.1016/j.molbiopara.2021.111431. Epub 2021 Nov 20.
Angiostrongylus cantonensis is a zoonotic parasitic nematode that is the most common cause of human eosinophilic meningitis. The invasive apple snail Pomacea canaliculata is an important intermediate host of A. cantonensis and contributes to its spread. P. canaliculata control will help prevent its invasion and transmission of A. cantonensis. The new molluscicide PBQ (1-(4-chlorophenyl)-3-(pyridin-3-yl)urea) exhibits great potency against P. canaliculata and has low toxicity against mammals and non-target aquatic organisms. We studied the mode of action of PBQ using TMT-based comparative quantitative proteomics analysis between PBQ-treated and control P. canaliculata snails. A total of 3151 proteins were identified, and 245 of these proteins were significantly differentially expressed with 135 downregulated and 110 upregulated. GO and KEGG enrichment analyses identified GO terms and KEGG pathways involved in de novo purine biosynthesis, ribosome components and translation process were significantly enriched and downregulated. The results indicated that PBQ treatment had substantial effects on the synthesis of genetic material, translation process, and protein synthesis of P. canaliculata and were likely the main cause of snail mortality.
广东住血线虫是一种人畜共患的寄生性线虫，是人类嗜酸性脑膜炎的最常见病因。入侵性苹果螺 Pomacea canaliculata 是广东住血线虫的重要中间宿主，有助于其传播。控制 P. canaliculata 将有助于防止其入侵和传播 A. cantonensis。新型软体动物杀虫剂 PBQ（1-(4-氯苯基)-3-(吡啶-3-基)脲）对 P. canaliculata 具有强大的杀伤力，对哺乳动物和非靶标水生生物的毒性较低。我们使用 TMT 基于比较定量蛋白质组学分析研究了 PBQ 的作用模式，比较了 PBQ 处理和对照 P. canaliculata 蜗牛之间的蛋白质表达。共鉴定出 3151 种蛋白质，其中 245 种蛋白质表达显著差异，其中 135 种下调，110 种上调。GO 和 KEGG 富集分析确定了参与从头嘌呤生物合成、核糖体成分和翻译过程的 GO 术语和 KEGG 途径显著富集和下调。结果表明，PBQ 处理对 P. canaliculata 的遗传物质合成、翻译过程和蛋白质合成有重大影响，可能是导致蜗牛死亡的主要原因。
