Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang524088, People's Republic of China.
Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang524088, People's Republic of China.
Br J Nutr. 2022 Nov 14;128(9):1674-1688. doi: 10.1017/S0007114521004670. Epub 2021 Nov 24.
The present study investigated the effect of black soldier fly () larvae meal (BSF) on haemolymph biochemical indicators, muscle metabolites as well as the lipid and glucose metabolism of Pacific white shrimp . Four diets were formulated in which the control diet contained 25 % of fishmeal (FM) and 10 % (BSF10), 20 % (BSF20), and 30 % (BSF30) of FM protein were replaced with BSF. Four hundred and eighty shrimp (0·88 ± 0·00 g) were distributed to four groups of three replicates and fed for 7 weeks. Results showed that growth performance of shrimp fed BSF30 significantly decreased compared with those fed FM, but there was no significant difference in survival among groups. The whole shrimp crude lipid content, haemolymph TAG and total cholesterol were decreased with the increasing BSF inclusion. The results of metabolomics showed that the metabolite patterns of shrimp fed different diets were altered, with significant changes in metabolites related to lipid metabolism, glucose metabolism as well as TCA cycle. The mRNA expressions of , , , , , , and in hepatopancreas were downregulated in shrimp fed BSF30, but mRNA expression of was upregulated. Unlike BSF30, the mRNA expressions of , , and in hepatopancreas were upregulated in shrimp fed BSF20. This study indicates that BSF20 diet promoted lipid synthesis and lipolysis, while BSF30 diet weakened -oxidation and glycolysis as well as affected the unsaturated fatty acids synthesis, which may affect the growth performance and body composition of shrimp.
本研究调查了黑水虻幼虫粉（BSF）对凡纳滨对虾血淋巴生化指标、肌肉代谢物以及脂类和葡萄糖代谢的影响。设计了 4 种饲料配方，对照组饲料中含有 25%的鱼粉（FM）和 10%的 BSF（BSF10），用 BSF 分别替代 20%（BSF20）和 30%（BSF30）的 FM 蛋白。将 480 只（0.88±0.00 g）虾随机分配到 4 个 3 重复组中，饲养 7 周。结果表明，与 FM 组相比，BSF30 组虾的生长性能显著降低，但各组间存活率无显著差异。随着 BSF 含量的增加，整虾粗脂肪含量、血淋巴 TAG 和总胆固醇均降低。代谢组学结果表明，不同饲料组虾的代谢物模式发生了改变，与脂类代谢、葡萄糖代谢以及 TCA 循环相关的代谢物发生了显著变化。BSF30 组虾肝胰腺中 、 、 、 、 、 和 的 mRNA 表达下调，但 基因的 mRNA 表达上调。与 BSF30 不同，BSF20 组虾肝胰腺中 、 、 和 的 mRNA 表达上调。本研究表明，BSF20 饲料促进了脂肪的合成和脂肪分解，而 BSF30 饲料减弱了β-氧化和糖酵解，影响了不饱和脂肪酸的合成，这可能影响虾的生长性能和体组成。
