Lu Yan, Dai Jun, Liu Qiyang, Cai Pan, Zheng Jianghua
Department of Laboratory Medicine, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, P.R. China.
Department of Orthopedic Surgery, Shanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai 201318, P.R. China.
Exp Ther Med. 2022 Jan;23(1):18. doi: 10.3892/etm.2021.10940. Epub 2021 Oct 30.
Pulmonary thromboembolism (PTE) is a fatal clinical syndrome that usually occurs in elderly individuals. The present study aimed to identify functional and key genes involved in the early diagnosis of PTE using bioinformatics analysis. The GSE84738 dataset was retrieved from the Gene Expression Omnibus database. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were subsequently performed. In addition, Cytoscape software v.3.7.2 was used to construct a protein-protein interaction (PPI) network. Serum samples from patients with PTE and healthy individuals were collected and the expression levels of Toll-like receptor (TLR)4, TLR2, IL-1β, JUN, prostaglandin-endoperoxide synthase 2 (PTGS2), osteopontin (SPP1) and endothelin-1 (ET-1) were analyzed by reverse transcription-quantitative PCR. A total of 160 upregulated and 159 downregulated differentially expressed genes were identified between patients with PTE and healthy individuals. TNF, IL-1β, JUN, TLR4, PTGS2, vascular cell adhesion molecule 1, SPP1, ryanodine receptor 2, TLR2 and ET-1 were considered as hub genes, which are defined as the genes with the highest degree of interaction in the enrichment and PPI network analyses. The top 10 common genes with the highest degree in the PPI network and the top 10 genes in modules 1 and 2 were TLR4, TLR2, IL-1β, JUN, PTGS2, SPP1 and ET-1. Taken together, the present study suggested that TLR4, TLR2, IL-1β and SPP1 were enriched in patients with PTE, thus providing novel potential biomarkers for the diagnosis of PTE.
肺血栓栓塞症（PTE）是一种通常发生在老年人中的致命性临床综合征。本研究旨在通过生物信息学分析确定参与PTE早期诊断的功能基因和关键基因。从基因表达综合数据库中检索GSE84738数据集。随后进行基因本体论和京都基因与基因组百科全书通路富集分析。此外，使用Cytoscape软件v.3.7.2构建蛋白质-蛋白质相互作用（PPI）网络。收集PTE患者和健康个体的血清样本，通过逆转录定量PCR分析Toll样受体（TLR）4、TLR2、白细胞介素-1β（IL-1β）、JUN、前列腺素内过氧化物合酶2（PTGS2）、骨桥蛋白（SPP1）和内皮素-1（ET-1）的表达水平。在PTE患者和健康个体之间共鉴定出160个上调和159个下调的差异表达基因。肿瘤坏死因子（TNF）、IL-1β、JUN、TLR4、PTGS2、血管细胞黏附分子1、SPP1、兰尼碱受体2、TLR2和ET-1被视为枢纽基因，其被定义为在富集和PPI网络分析中具有最高相互作用程度的基因。PPI网络中度数最高的前10个常见基因以及模块1和2中的前10个基因是TLR4、TLR2、IL-1β、JUN、PTGS2、SPP1和ET-1。综上所述，本研究表明TLR4、TLR2、IL-1β和SPP1在PTE患者中富集，从而为PTE的诊断提供了新的潜在生物标志物。
