Mititelu Radu Razvan, Albu Carmen Valeria, Bacanoiu Manuela Violeta, Padureanu Vlad, Padureanu Rodica, Olaru Gabriela, Buga Ana-Maria, Balasoiu Maria
Department of Microbiology, University of Medicine and Pharmacy of Craiova, Romania.
Department of Neurology, University of Medicine and Pharmacy of Craiova, Romania.
Discoveries (Craiova). 2021 Sep 28;9(3):e135. doi: 10.15190/d.2021.14. eCollection 2021 Jul-Sep.
Multiple sclerosis (MS) is a progressive and irreversible disease which affects the central nervous system (CNS) with still unknown etiology. Our study aimes to establish the homocysteine pattern that can predict the MS diseases progression and to identify a potential disease progression marker that can be easy to perform and non-invasive, in order to predict the diseases outcome. In order to achieve this goal, we included 10 adult RRMS subjects, 10 adult SPMS subjects and 10 age-matched healthy subjects. The homocysteine plasma level was measured using automated latex enhanced immunoassay and the cobalamin and folate measurements were performed using automated chemiluminescence immunoassay (CLIA). HCR was calculated by dividing the homocysteine plasma level by cobalamin plasma level. We found that the homocysteine level in plasma of both RRMS patients and SPMS group are significantly increased compared with the control group. There is a significantly higher concentration of homocysteine in SPMS group compared with the RRMS group. In addition, the HCR is significantly increased in SPMS compared with the RRMS group and is a very good index of disease severity.
多发性硬化症（MS）是一种影响中枢神经系统（CNS）的进行性且不可逆的疾病，其病因尚不明确。我们的研究旨在建立能够预测MS疾病进展的同型半胱氨酸模式，并确定一种易于实施且非侵入性的潜在疾病进展标志物，以预测疾病的转归。为实现这一目标，我们纳入了10名成年复发缓解型多发性硬化症（RRMS）患者、10名成年继发进展型多发性硬化症（SPMS）患者以及10名年龄匹配的健康受试者。使用自动乳胶增强免疫测定法测量血浆同型半胱氨酸水平，使用自动化学发光免疫测定法（CLIA）进行钴胺素和叶酸的测量。同型半胱氨酸与钴胺素比值（HCR）通过将血浆同型半胱氨酸水平除以血浆钴胺素水平来计算。我们发现，RRMS患者组和SPMS组血浆中的同型半胱氨酸水平与对照组相比均显著升高。与RRMS组相比，SPMS组的同型半胱氨酸浓度显著更高。此外，与RRMS组相比，SPMS组的HCR显著升高，并且是疾病严重程度的一个很好指标。
