Safari Dodi, Putri Hanifah Fajri Maharani, Bimantari Arrayan, Paramaiswari Wisiva Tofriska, Tafroji Wisnu, Khoeri Miftahuddin Majid, Salsabila Korrie
Eijkman Institute for Molecular Biology, Jakarta, Indonesia.
Biology Program, Surya University, Tangerang, Indonesia.
Access Microbiol. 2021 Oct 25;3(10):000271. doi: 10.1099/acmi.0.000271. eCollection 2021.
We investigated the genetic variation of and genes encoding dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR) enzymes amongst trimethoprim/sulfamethoxazole (co-trimoxazole) non-susceptible isolated from the Indonesian population. Archived isolates were screened for the presence and analysis of and genes using the polymerase chain reaction sequencing method. We found that 80 % of co-trimoxazole non-susceptible isolates (=30/39) showed a 6 bp insertion in the sulphonamide-binding site of DHPS. The Asp-92-Ala and Ile-100-Leu substitutions were more common on DHFR (42 %; 22/53) followed by the Asp-92-Ala, Glu-94-Asp and Ile-100-Leu substitutions (32 %; 17/53). The combination of the Ile-100-Leu substitution at the DHFR region and the 6 bp insertion was the most dominant combination among isolates having both and genes.
我们研究了从印度尼西亚人群中分离出的对甲氧苄啶/磺胺甲恶唑（复方新诺明）不敏感的[具体微生物名称未给出]中，编码二氢蝶酸合酶（DHPS）和二氢叶酸还原酶（DHFR）的[具体基因名称未给出]和[具体基因名称未给出]基因的遗传变异情况。使用聚合酶链反应测序方法，对存档的[具体微生物名称未给出]分离株进行筛选，以检测[具体基因名称未给出]和[具体基因名称未给出]基因的存在并进行分析。我们发现，80%的对复方新诺明不敏感的分离株（30/39）在DHPS的磺胺结合位点有一个6bp的插入。Asp-92-Ala和Ile-100-Leu替换在DHFR上更为常见（42%；22/53），其次是Asp-92-Ala、Glu-94-Asp和Ile-100-Leu替换（32%；17/53）。在同时具有[具体基因名称未给出]和[具体基因名称未给出]基因的分离株中，DHFR区域的Ile-100-Leu替换与6bp插入的组合是最主要的组合。
