重组大肠杆菌细胞裂解物作为兽医梭菌病疫苗的低成本替代品。
Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases.
机构信息
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Institut fϋr Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Spielmannstr, Technische Universität Braunschweig, Braunschweig, Germany.
出版信息
Methods Mol Biol. 2022;2411:105-115. doi: 10.1007/978-1-0716-1888-2_6.
This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.
本章描述了一种实用、适合行业需求且高效的疫苗方案，该方案基于使用含有重组抗原的大肠杆菌细胞成分（包含体或细胞裂解上清液）。通过在实验室和农场动物中进行小鼠血清中和试验对该方法进行了特征描述和评估，结果表明，该方法是诱导针对梭菌病的保护性免疫应答的一种极好的替代方法。
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