梭菌毒素：在大肠杆菌中表达和鉴定的实用指南。
Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli.
机构信息
Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil.
Institut fϋr Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie, Technische Universität Braunschweig, Braunschweig, Germany.
出版信息
Methods Mol Biol. 2022;2411:117-125. doi: 10.1007/978-1-0716-1888-2_7.
Farm animals are frequently affected by a group of diseases with a rapid clinical course, caused by Clostridium spp. and immunization is essential to provide protection. However, the current manufacturing platform for these vaccines has disadvantages and the main alternative is the use of an expression system that uses Escherichia coli to obtain recombinant vaccine antigens. In this chapter we describe procedures for cloning, expression and characterization of recombinant toxins from Clostridium spp. produced in E. coli for veterinary vaccine applications.
家畜常受到一组由梭菌引起的具有快速临床病程的疾病的影响，免疫接种对于提供保护至关重要。然而，目前这些疫苗的制造平台存在缺点，主要的替代方法是使用表达系统，利用大肠杆菌获得重组疫苗抗原。在本章中，我们描述了从大肠杆菌中生产的用于兽医疫苗应用的梭菌重组毒素的克隆、表达和特性分析的程序。
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