Food Safety and Microbial Physiology Lab, Faculty of Agriculture, 68779Mansoura University, Mansoura, Egypt.
Sci Prog. 2021 Oct;104(4):368504211055277. doi: 10.1177/00368504211055277.
Thermophilic strains of can express enzymes of higher thermal stability, which allows carrying out industrial fermentations under higher temperatures. This lowers the contamination potential, accelerates mixing rates and facilitates the recovery of fermentation end products. The present study was thus designed to isolate and characterize thermophilic cultures from soil and compost samples. Forty-two thermophilic isolates could be identified employing morphological, physiological and the 16S rRNA gene sequencing analyses. The isolates showed a high degree of biological diversity involving 13 species and 1 subspecies but were dominated by . Phylogenetic analysis of isolates based on the DNA sequencing of and genes presented them in two main genetic groups. Isolates of five thermophilic species including and showed multiple activities to degrade all of cellulose, hemicellulose and lignin. Those multifunctional thermophilic isolates can be harnessed in the degradation of plant wastes for the production of biofuels and compost.
可以从土壤和堆肥样本中分离和鉴定出嗜热 。本研究的目的就是从土壤和堆肥样本中分离和鉴定嗜热 。
采用形态学、生理学和 16S rRNA 基因测序分析，可鉴定出 42 株嗜热 。这些分离株表现出高度的生物多样性，涉及 13 个种和 1 个亚种，但以 为主。基于 和 基因 DNA 测序的 分离株的系统发育分析将它们分为两个主要的遗传群。包括 和 在内的 5 种嗜热种的分离株表现出对所有纤维素、半纤维素和木质素的多种降解活性。这些多功能嗜热 分离株可用于植物废物的降解，以生产生物燃料和堆肥。
