位于2号染色体短臂上的D2S1与ACP1之间存在非常紧密的连锁关系。

Very close linkage between D2S1 and ACP1 on chromosome 2p.

作者信息

Lothe R A, Gedde-Dahl T, Olaisen B, Bakker E, Pearson P

机构信息

Department of Genetics, Norwegian Radium Hospital, Oslo.

出版信息

Ann Hum Genet. 1986 Oct;50(4):361-7. doi: 10.1111/j.1469-1809.1986.tb01757.x.

DOI:10.1111/j.1469-1809.1986.tb01757.x

PMID:3481680

Abstract

The genomic DNA-probe L2.30 was used to assign D2S1 to 2p23-pter by in situ hybridization. The RFLP revealed by BglII was then used for linkage studies in the Oslo-NHIK families segregating for the acid phosphatase ACP1 protein polymorphism. Evidence for very close linkage was found by a lod score of +17.17 at recombination fraction theta = 0.01. By this close linkage 92 informative meioses could be inferred from the families and with only a single crossover. The upper probability limit to the recombination fraction is 0.06 according to the HGM 8 criterion. No association between ACP1 alleles and D2S1 BglII alleles was found. The Norwegian gene frequencies for D2S1 were A1 (9.0 kb) = 0.65 and A2 (6.3 kb) = 0.35.

摘要

基因组DNA探针L2.30通过原位杂交用于将D2S1定位于2p23 - pter。然后，由BglII揭示的限制性片段长度多态性（RFLP）用于在奥斯陆 - NHIK家族中进行连锁研究，这些家族正在分离酸性磷酸酶ACP1蛋白多态性。在重组分数θ = 0.01时，通过对数优势（lod）分数为 +17.17发现了非常紧密连锁的证据。通过这种紧密连锁，可以从家族中推断出92个信息性减数分裂，且只有一次交换。根据HGM 8标准，重组分数的上限概率为0.06。未发现ACP1等位基因与D2S1 BglII等位基因之间存在关联。D2S1在挪威的基因频率为A1（9.0 kb）= 0.65和A2（6.3 kb）= 0.35。