通过与DNA限制性片段长度多态性（DNA-RFLP）紧密连锁，基德（JK）血型位点被定位到18号染色体上。

The Kidd (JK) blood group locus assigned to chromosome 18 by close linkage to a DNA-RFLP.

作者信息

Geitvik G A, Høyheim B, Gedde-Dahl T, Grzeschik K H, Lothe R, Tomter H, Olaisen B

机构信息

Department of Genetics, Norwegian Radiumhospital, Oslo.

出版信息

Hum Genet. 1987 Nov;77(3):205-9. doi: 10.1007/BF00284470.

DOI:10.1007/BF00284470

PMID:2890568

Abstract

The close linkage between the PstI-restriction fragment length polymorphism (RFLP) disclosed by the L2.7 genomic DNA probe and the Kidd blood group locus is described. The maximum lod score is +8.53 at recombination fraction theta = 0.03. The upper probability limit of the recombination fraction is theta 1 = 0.11. The L2.7 probe, previously assigned provisionally to chromosome 17, is by the present study assigned to chromosome 18. This also assigns the Kidd blood group locus (JK) to chromosome 18. Accepting previous deletion mapping, the shortest regions of overlap (SRO) for JK is 18q11-12, whereas one of our hybrids assigns L2.7 to 18p11-pter, suggesting centromeric localisation of the linkage group. JK has been assigned previously to chromosome 2 because of its provisional linkage to IGK which in turn has been mapped to 2p12. Our own JK-IGK linkage data do in fact support the previous positive lod scores at high recombination fractions (total lods +4.12 at theta1 = 0.30). No obvious explanation for the conflicting gene mapping data is found.

摘要

本文描述了由L2.7基因组DNA探针揭示的PstI限制性片段长度多态性（RFLP）与基德血型位点之间的紧密连锁关系。在重组率θ = 0.03时，最大lod分数为+8.53。重组率的上限概率为θ1 = 0.11。先前暂时定位于17号染色体的L2.7探针，在本研究中被定位于18号染色体。这也将基德血型位点（JK）定位于18号染色体。根据先前的缺失定位，JK的最短重叠区域（SRO）为18q11 - 12，而我们的一个杂种细胞系将L2.7定位于18p11 - pter，提示连锁群的着丝粒定位。由于JK先前与IGK暂时连锁，而IGK又被定位于2p12，所以JK先前被定位于2号染色体。我们自己的JK - IGK连锁数据实际上确实支持先前在高重组率时的阳性lod分数（在θ1 = 0.30时，总lod为+4.12）。未发现对相互矛盾的基因定位数据的明显解释。