Department of Biology, College of Education, Salahaddin University-Erbil, Kurdistan Region, Iraq.
Cell Mol Biol (Noisy-le-grand). 2021 Aug 31;67(2):171-177. doi: 10.14715/cmb/2021.67.2.27.
Type 2 diabetes mellitus (T2DM) is a metabolic disease and cytokines show a vital role in the T2DM progress. The goal of this research was to assess serum levels of tumor necrosis factor-alpha (TNF-?) and interferon-gamma (IFN-?) gene polymorphism in T2DM in Kurdish patients. Levels of serum IFN-? and TNF-? were assessed through enzyme-linked immune sorbent assay in individuals with T2DM and the control group. DNA was extracted and the amplification refractory mutational system method was utilized for genotyping the IFN-? (+874) A/T and TNF-? (-308) G/A. The Hardy-Weinberg equilibrium was evaluated with the ?2-test. The IFN- ? serum levels were significantly different between patients with T2DM and control individuals (P<0.05). But the C-reactive protein (CRP) and TNF-? serum levels were not significantly different between them (P>0.05). The serum level of IFN- ? (+874) AT genotype and TNF-? (- 308) GG genotypes were significantly higher in the T2DM group comparing with healthy people (P<0.05). A significant relation between T2DM and IFN-? (+874) gene polymorphism's TT and AT genotypes was observed. Also, it was not a significant relation between TNF-? (?308) gene polymorphism's GG and GA genotypes and T2DM. But the statistically significant difference was found in the genotype AA frequency. Genetic polymorphisms of IFN-? (+874) and TNF-? (?308) are contributed to the genetic susceptibility for T2DM development in the Kurdish population. Early screening of these two genetic polymorphisms may assist in the early control and management of T2DM.
2 型糖尿病（T2DM）是一种代谢性疾病，细胞因子在 T2DM 进展中发挥着重要作用。本研究旨在评估库尔德人群中 2 型糖尿病患者血清肿瘤坏死因子-α（TNF-?）和干扰素-γ（IFN-?）基因多态性水平。通过酶联免疫吸附试验（ELISA）检测 T2DM 患者和对照组个体的血清 IFN-?和 TNF-?水平。提取 DNA 后，采用扩增受阻突变系统（ARMS）方法对 IFN-?（+874）A/T 和 TNF-?（-308）G/A 进行基因分型。采用卡方检验（?2-test）评估 Hardy-Weinberg 平衡。T2DM 患者和对照组个体的 IFN-?血清水平存在显著差异（P<0.05）。但是，C 反应蛋白（CRP）和 TNF-?血清水平在两组之间没有显著差异（P>0.05）。与健康人群相比，T2DM 组的 IFN-?（+874）AT 基因型和 TNF-?（-308）GG 基因型的血清水平显著升高（P<0.05）。T2DM 与 IFN-?（+874）基因多态性的 TT 和 AT 基因型之间存在显著相关性。此外，TNF-?（-308）基因多态性的 GG 和 GA 基因型与 T2DM 之间不存在显著相关性。但是，AA 基因型的频率存在统计学差异。IFN-?（+874）和 TNF-?（-308）的遗传多态性与库尔德人群 2 型糖尿病的遗传易感性有关。对这两种遗传多态性的早期筛查可能有助于 2 型糖尿病的早期控制和管理。
