Department of Pathology, Liyang Branch, Jiangsu People's Hospital, Liyang, Jiangsu, 213300, China.
Department of General Surgery, Liyang Branch, Jiangsu People's Hospital, Liyang, Jiangsu, 213300, China.
Cell Mol Biol (Noisy-le-grand). 2021 Aug 31;67(2):161-165. doi: 10.14715/cmb/2021.67.2.25.
Forkhead box (FOX) transcription factors regulate the development of several human cancers. However, the role and therapeutic potential of FOXA1 in gastric cancer is still largely unexplored. The results showed a significant (P < 0.05) upregulation of FOXA1 in gastric cancer tissues and cell lines. Silencing of FOXA1 in gastric cells significantly (P < 0.05) decreased their viability through induction of apoptosis. The induction of apoptosis was associated with upregulation of Bax and downregulation of Bcl-2. Additionally, FOXA1 silencing caused activation of caspase-3 and 9 with no apparent effects on the expression of caspase-8 suggestive of intrinsic apoptosis. The transwell cell invasion revealed significant (P < 0.05) decline of cell invasion of gastric cancer cells upon FOXA1 silencing. The FOXA1 knockdown further inhibited the in vivo tumor growth suggestive of its therapeutic potential. Taken together, the findings of the present revealed that FOXA1 regulates the proliferation and development of gastric cancer and may exhibit therapeutic implications in gastric cancer treatment.
叉头框（FOX）转录因子调节几种人类癌症的发展。然而，FOXA1 在胃癌中的作用和治疗潜力在很大程度上仍未得到探索。结果表明，FOXA1 在胃癌组织和细胞系中显著上调（P<0.05）。FOXA1 在胃细胞中的沉默通过诱导细胞凋亡显著（P<0.05）降低其活力。细胞凋亡的诱导与 Bax 的上调和 Bcl-2 的下调有关。此外，FOXA1 沉默导致 caspase-3 和 caspase-9 的激活，而 caspase-8 的表达无明显变化，提示存在内在凋亡。Transwell 细胞侵袭实验显示，FOXA1 沉默后胃癌细胞的侵袭明显下降（P<0.05）。FOXA1 敲低进一步抑制体内肿瘤生长，提示其具有治疗潜力。综上所述，本研究结果表明，FOXA1 调节胃癌的增殖和发展，可能在胃癌治疗中具有治疗意义。
